摘要
为了探讨Ghrelin对绵羊早期胚胎发育的影响。利用绵羊体外受精技术制备绵羊早期胚胎,并分别将受精胚胎放置在4组培养液中进行发育培养:①梯度添加300、600、1 000、4 000、10 000ng·mL-1 Ghrelin的培养液;②梯度添加1、10、200、400ng·mL-1 Ghrelin受体拮抗剂培养液;③含400ng·mL-1 Ghrelin受体拮抗剂且梯度添加300、1 000、4 000ng·mL-1 Ghrelin的培养液;④空白对照组。在胚胎受精第2、3、4、5天时观察胚胎发育情况,分别统计绵羊2-细胞、4-细胞、8-细胞和16-细胞胚胎的卵裂率。结果显示:与空白对照组比较,在培养液中添加不同浓度的Ghrelin的体外受精胚胎的卵裂率均无显著变化(P>0.05);在培养液中梯度添加Ghrelin受体拮抗剂,绵羊2-细胞、16-细胞胚胎的卵裂率无明显变化(P>0.05),但4-细胞、8-细胞胚胎的卵裂率显著降低,且在200、400ng·mL-1时差异极显著(P<0.01);在含受体拮抗剂且梯度添加Ghrelin组,4-细胞、8-细胞胚胎卵裂率有所恢复,但与空白对照组比较差异显著(P<0.05)。结果表明:Ghrelin对绵羊4-细胞、8-细胞胚胎发育具有促进作用。
In order to investigate the effects of Ghrelin on the development of early ovine embryos.Early ovine embryos were prepared by in vitro fertilization.The fertilized embryos were cultured in four groups of culture medium:①The group of adding gradiently 300,600,1 000,4 000,10 000ng·mL-1 Ghrelin;②The group of adding gradiently 1,10,200,400ng·mL-1 Ghrelin receptor antagonist;③The group of containing 400ng·mL-1 Ghrelin receptor antagonist and adding gradiently 300,1 000,4 000ng·mL-1 Ghrelin;④The control group.Embryo development was observed on the 2nd,3rd,4th,5th day,and the cleavage rates of ovine embryos were calculated at 2-cell,4-cell,8-cell and 16-cell stages.The results showed that:Compared with control group,ovine embryo cleavage rates had no significant change in the group adding Ghrelin(P>0.05);Embryo cleavage rate had no obvious change at 2-cell,16-cell stages(P>0.05),butdecreased significantly at 4-cell,8-cell stages,and extremely significant difference at 200,400ng·mL-1 in the group adding Ghrelin receptor antagonist(P<0.01);Embryo cleavage rates was recovered slowly on 4-cell,8-cell stages by gradiently adding Ghrelin but still significant difference in the group containing Ghrelin receptor antagonist(P<0.05).These results indicate:Ghrelin promote ovine embryo development at 4-cell and 8-cell stages.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2014年第6期926-931,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金项目(30860201)
