摘要
目的:对菲律宾蛤仔糖胺聚糖RG-1的一级结构特征及细胞免疫调节活性进行研究。方法:采用高效液相色谱法测定相对分子质量和单糖组成,通过红外光谱、高碘酸氧化和Smith降解等研究其糖链主要结构特征;用MTT法分析对小鼠脾淋巴细胞的影响。结果:RG-1主要由葡萄糖、氨基半乳糖和半乳糖通过1→6、1→4或1→4,6键连接构成主链,在主链中还含有大量的半乳糖醛酸,重均相对分子质量为9.94×105。RG-1具有显著增强小鼠腹腔巨噬细胞吞噬中性红的作用和脾淋巴细胞的增殖作用(p<0.01)。结论:菲律宾蛤仔糖胺聚糖可通过增强细胞免疫活性而起到抗肿瘤作用。
Objective： To investigate the structural characterstics and cells immunomodulatory activity of the glycosaminoglycan RG-1 from Ruditapes philippinarum. Methods： The morlicular weight and monosaccharide composition of the glycosaminoglycan RG-1 was analysed by HPGPC, its main structure characterstics was studied by IR, periodate oxidation and Smith degradation. Effects of RG-1 on proliferation of KM mouse spleen lymphocytes were detected by MTT. Results： RG-1 was composed of Glc-GalNAc-Gal by 1→6,1→4 or 1→4,6 in main chain backbone which contained much galacturonic acid with Mr 9.94×105. RG-1 significantly simulated the phagocytic activity of mice peritoneal macrophages and proliferation of KM mouse spleen lymphocytes in vitro （p〈0.01）. Conclusion：N Glycosaminoglycan RG-1 can inhibit the cancer by enhance cell immunological activity.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2012年第2期186-190,共5页
Journal of Chinese Institute Of Food Science and Technology
关键词
菲律宾蛤仔
糖胺聚糖
结构分析
免疫调节
Ruditapes philippinarum
glycosaminoglycan（RG-1 ）
structural analysis
immunoregulation
