摘要
目的建立基于简并寡核苷酸引物PCR(degenerate oligonucleotide primed-PCR,DOP-PCR)的全基因组检测体系,并对其可靠性、灵敏度进行研究。方法通过荧光标记STR复合扩增毛细管电泳检测系统,对DOP-PCR扩增产物进行检测,获得DOP-PCR检测体系的灵敏度、可靠性。结果成功建立了可靠的DOP-PCR检测体系,经过DOP-PCR预处理再进行STR分型检验,其检测灵敏度可达到5个细胞量(相当于30 pg)。结论本研究建立的DOP-PCR技术可靠且可提高法医学微量检材的检测灵敏度。
Objective To establish a whole genome amplification testing system based on degenerate oligonucleotide primed-PCR（DOP-PCR） and to explore its reliability and sensitivity.Methods DOP-PCR amplified production was detected by fluorescent labeled multiplex STR amplification and capillary electrophoresis detection system to determine reliability and sensitivity of DOP-PCR system.Results DOP-PCR system was successfully established and the detection sensitivity reached 5 cells（30 pg） by pretreatment of DOP-PCR and then detection of STR genotyping.Conclusion The system established in this study is reliable and more testing sensitive for forensic trace evidence.
出处
《法医学杂志》
CAS
CSCD
2012年第1期41-43,共3页
Journal of Forensic Medicine
基金
国家自然科学基金资助项目(30271446)
高校博士点专项科研基金资助项目(20020610044)
关键词
法医遗传学
基因扩增
简并寡核苷酸引物PCR
forensic genetics
gene amplification
degenerate oligonucleotide primed-PCR
