摘要
目的运用多位点STR复合扩增技术进行亲权鉴定,探讨Identifiler Plus及Goldeneye 20A试剂盒在突变性亲权鉴定或STR异常分型中的作用。方法用Identifiler Plus及Goldeneye 20A试剂盒对亲权鉴定中的样本DNA进行PCR扩增、扩增产物进行毛细管电泳,应用Gene Mapper v3.2软件基因分型,最后进行亲权关系的鉴定。结果两种试剂盒的扩增产物共同的16个STR基因座分型一致,在D18S51基因座均出现STR分型异常,从而确认D18S51基因座为异常三带型。由于本亲权鉴定中共有5个基因座不符合孟德尔遗传定律,故排除单亲遗传关系。结论 Identifiler Plus及Goldeneye 20A两种试剂盒在基因突变或STR分型异常的亲权鉴定案例中可以起到相互佐证、补充的作用。
Objective The mutational or abnormal genotyping of STR loci in parentage testing was per- formed by using Identifiler-Plus and Goldeneye 20A kits. Methods The paternity testing cases was amplified by the Identifiler Plus and Goldeneye 20A kits. The amplified products were detected by capillary electropho- resis, and the genotypes were analyzed by GeneMapper v3.2. Results The amplified products had the same abnormal genotyping on D18S5l STR loci using two kinds of kits. According to the laws of inheritance ,the results of exclusive paternity were in coordinate with more than 3 loci. Conclusion The experiments suggest that two kinds of kits can support each other in the mutational or abnormal genotyping of STR loci in parent- age testing cases.
出处
《济宁医学院学报》
2016年第4期267-270,共4页
Journal of Jining Medical University
基金
济宁医学院科研计划项目(JY2015KJ006)
济宁市科技发展计划项目(2015-85)
山东省自然科学项目(ZR2014HQ018)
上海市法医学重点实验室开放课题(KF1510)
