摘要
在不同氮(N)、磷(P)初始浓度的培养液中对铜绿微囊藻(Microcystis aeruginosa)进行培养.利用Monod方程分别计算铜绿微囊藻对氮、磷的半饱和常数(Ks).结果表明:以单一元素为限制底物时,满足铜绿微囊藻正常生长的氮浓度大于4.0 mg·L^(-1),磷浓度大于0.50 mg·L^(-1);铜绿微囊藻最适生长的氮浓度范围为32.0～64.0 mg·L^(-1),磷浓度范围为1.0～1.50 mg·L^(-1);以磷为限制底物时的半饱和常数Ks_p。远远小于以氮为限制底物时的半饱和常数Ks_N(Ks_N>Ks_p),说明铜绿微囊藻对磷的亲和性高于氮.与铜绿微囊藻最大现存量(X)呈高度线性相关时的氮浓度范围为0.20～64.0 mg·L^(-1),磷浓度范围为0.02～1.50 mg·L^(-1).铜绿微囊藻比增长速率(μ)连续增加的氮浓度范围为0.20～1.60 mg·L^(-1),磷浓度范围为0.02～0.50 mg·L^(-1).
Microystis aeruginosa was cultured in solutions of different initial nitrogen and phos- phorus concentrations. The semisaturation constants （Ks） of Microystis aeruginosa to P and N were calculated respectively by using the Monod equation. The results show that the Microystis aeruginosa grew normally when the concentrations of N and P were greater than 4.0 mg - L^-1and 0.50 mg - L^-1 respectively. The optimum concentration ranges of N and P for Microystis aeruginosa growth were 32.0~64.0 mg - L^-1 and 1.0-1. 50 mg ~ L 1 respectively. KSN is higher than Ksp, which indicates that the affinity of P to Microystis aeruginosa is higher than N.When the concentration ranges of N and P were 0.20-64.0 mg - L^-1 and 0.02-1. 50 mg - L respectively, they had high liner correlation with the largest extent quantity of Microystis aeruginosa. The special growth rate increases continuously when the concentration ranges of N and P are 0.20-1.60 ragL ^-1 and 0. 02-0. 50 rag- L^-1respectively.
出处
《华东师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第1期11-18,共8页
Journal of East China Normal University(Natural Science)
基金
青草沙水库藻类变化规律与生物预控关键技术研究(09DZ120010A)
关键词
铜绿微囊藻
氮
磷
生长特性
Microcystis aeruginosa
nitrogen
phosphorus
growth characteristics
