摘要
建立了一种基于纯水提取的新型烟熏鲣鱼中肌苷酸的提取方法。样品前处理采用95℃的纯水作为提取液,提取5 min。采用的色谱条件:色谱柱为Agilent Zorbax SB-C18柱(150 mm×4.6 mm,5μm),柱温为30℃,流动相为甲醇-50 mmol/L乙酸铵水溶液(5∶95,v/v),流速为1.0 mL/min,检测波长为254 nm。结果表明,该方法测定烟熏鲣鱼中肌苷酸含量的线性范围为1.0～120.0 mg/L;在0.40、2.00、4.00 g/kg(n=3)的添加水平下,回收率为84.6%～100.2%,测定结果的相对标准偏差(RSD)为1.00%～6.12%;检出限(信噪比(S/N)为3)为4 mg/kg;定量限(S/N=10)为12 mg/kg。该方法与常规采用的基于高氯酸的提取方法相比较,提取效率提高了37.0%,并且该方法操作安全、与环境友好,对烟熏鲣鱼中肌苷酸含量测定准确度良好,对其他食品中肌苷酸含量的测定具有借鉴意义,是一种值得推广的样品预处理方法。
A novel method for the extraction of inosinic acid in Smoked Katsuwonus pelamis based on water was developed. The inosinic acid was extracted by 95 ℃ water for 5 min. The subsequent analysis of inosinic acid was achieved on an Agilent Zorbax SB-C18 column （150 mm×4.6 mm, 5 μm） with the mobile phase of methanol-50 mmol/L ammonium acetate （5∶95, v/v） at a flow rate of 1.0 mL/min at 30 ℃. The detection wavelength was set at 254 nm. The results showed that the linear range for inosinic acid in Smoked Katsuwonus pelamis was between 1.0 mg/L and 120.0 mg/L with the limit of detection （LOD, S/N=3） of 4 mg/kg and the limit of quantification （LOQ, S/N=10） of 12 mg/kg. At the spiked levels of 0.40, 2.00, 4.00 g/kg （n=3）, the recoveries ranged from 84.6% to 100.2% with the relative standard deviations （RSDs） between 1.00% and 6.12% . Compared with traditional extraction method by perchloric acid, the extraction efficiency was improved by 37.0% . The novel method is safe, stable and accurate for the determination of inosinic acid in Smoked Katsuwonus pelamis, which can also be applied to the determination of inosinic acid in other foods and deserved to be spread.
出处
《色谱》
CAS
CSCD
北大核心
2012年第2期215-218,共4页
Chinese Journal of Chromatography
基金
宁波工程学院科研启动基金(No.0080011540054)
关键词
纯水提取
高效液相色谱
肌苷酸
烟熏鲣鱼
water extraction
high performance liquid chromatography （HPLC）
inosinic acid
Smoked Katsuwonus pelamis
