摘要
采用常规压片法,首次对甘肃康乐地产野生百里香染色体进行核型分析。结果表明,上午8:45-9:15取材,0.04%秋水仙素和0.002mol/L 8-羟基喹啉溶液等体积混合液处理百里香芽尖2~3h,改良石炭酸品红染色液染色1h效果最好;对百里香体细胞染色体数目进行统计,核型分析结果表明,百里香的细胞染色体数为2n=24,其中中部染色体(m)为10对,近中部着色点染色体(sm)2对,其核型公式为2n=2x=24=20m+4sm,核型属2A型,核型不对称系数为59.63%。
The karyotype of Thymus mongolicus was analyzed using a chromosome mounting technique.The best sampling time was between 8:45 am and 9:15 am,the best pre-treatment was 0.04% colchicine and 0.002 mol/L 8-hydroxyquinoline mixture(1∶1) for 2-3 h,and the dyeing treatment was 1 h with modified carbolic acid-magenta.Karyotype analysis of T.mongolicus showed that the chromosome number is 24 and the karyotype formula is 2n=2x=24=20m+4sm.Ten pairs are metacentric(m) and 2 pairs are submetacentric(sm).The karyotype of T.mongolicus is 2A and the karyotype asymmetry coefficient is 59.63%.
出处
《草业学报》
CSCD
北大核心
2012年第1期184-189,共6页
Acta Prataculturae Sinica
基金
国家自然科学基金(31160087)
甘肃省自然科学基金(0803RJ ZA038)
甘肃省教育厅研究生导师项目(1101-06)
甘肃省农牧厅(GYC09-10)
西北师范大学科技与知识创新基金(NWNU-KJCXGC-03-58)资助
关键词
百里香
染色体
核型分析
Thymus mongolicus
chromosome
karyotype analysis