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牡丹沙培嫩茎段离体培养的初步研究

Preliminary Study on in Vitro Culture of Sand-cultured Tender Stem Segments of Paeonia suffruticosa
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摘要 以牡丹沙培嫩茎段为外植体,进行了离体培养的初步研究。结果表明:牡丹沙培嫩茎段诱导培养接种的最佳培养基为改良B5+BA 1.0mg/L+NAA 0.05mg/L,最佳的光照时数为16h/d;牡丹增殖培养时在基本培养中添加BA 1.5mg/L时增殖效果最好。 With sand-cultured tender stem segments of Paeonia suffruticosa as explants,the preliminary culture in vitro was studied. The results showed that the induced culture section of Paeonia suffruticosa sand-cultured tender stem segments' best medium for improved vaccination Bs +BA 1. 0 mg/L+NAA 0. 05 mg/L,the best light hours was 16 h/d; Paeonia suffruticosa proliferation in cultured basic training in the concentration of added BA 1.5 mg/L proliferation best.
作者 关丽霞
出处 《北方园艺》 CAS 北大核心 2012年第4期74-75,共2页 Northern Horticulture
关键词 牡丹 茎段 离体培养 Paeonia su f fruticosa stem in vitro culture
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