摘要
目的应用中空纤维超滤技术纯化脱毒后的无细胞百日咳抗原。方法采用中空纤维超滤法纯化脱毒后的无细胞百日咳抗原,并从纯化样品的纯度、回收率及效价等方面与透析法进行对比。结果超滤法去除无细胞百日咳抗原溶液中脱毒剂和色素的效率更高(3 h内即可完成),且所得原液的回收率较高(92.1%),效价(11.166 IU/ml)也高于透析法(9.211 IU/ml)。结论应用中空纤维超滤技术纯化无细胞百日咳抗原可替代透析工艺。
Objective To purify detoxified acellular pertussis antigen by hollow fiber ultrafiltration. Methods The acellular pertussis antigen after detoxification was purified by hollow fiber ultrafiltration, of which the purity, recovery rate and titer were com- pared with those purified by dialysis. Results Hollow fiber uhrafihration was highly effective in removal of detoxifying agent and pig- ment in acellular pertussis antigen solution, which was completed within 3 h. The recovery rate of purified bulk was 92. 1%, while the titer （11. 166 IU/ml） was higher than that purified by dialysis （9. 211 IU/ml）. Conclusion Hollow fiber uhrafitration may be used for purification of acellular pertussis antigen instead of dialysis.
出处
《中国生物制品学杂志》
CAS
CSCD
2012年第2期241-242,共2页
Chinese Journal of Biologicals
基金
"重大新药创制"科技重大专项(2011zx09201-301
2011zx-09401-302)
关键词
超滤
百日咳
中空纤维
Ultrafiltration
Pertussis
Hollow fiber
