摘要
目的探讨miR-92a反义寡核苷酸(ASO)对胃癌细胞增殖和凋亡的影响。方法将胃癌细胞SGC-7901和MKN-45分为反义寡核苷酸处理组和无义寡核苷酸对照组,分别转染miR-92a ASO和无义寡核苷酸,同时设未转染空白对照组;实时荧光定量PCR(qRT-PCR)检测处理前以及处理48 h后胃癌细胞中miR-92a的表达变化;MTT法检测细胞增殖情况;AnnexinV-FITC/PI双染色流式细胞术和Hoechst33258荧光染色观察细胞凋亡情况。结果 Anti-miR-92a作用48 h后,miR-92a在两种胃癌细胞中的表达均显著下降(P<0.05);细胞增殖受到明显抑制(P<0.05);细胞凋亡率明显增加(P<0.05),荧光染色显示其细胞核呈凋亡细胞的特征样改变。结论以miR-92a为靶标的ASO可以有效抑制胃癌细胞增殖,促进其凋亡。
Objective To study the effect of anti-miR-92a oligonucleotides on proliferation and apoptosis of gastric cancer cell lines.Methods Gastric cancer cell lines,SGC-7901 and MKN-45,were cultured in vitro and transfected with anti-miR-92a oligonucleotides.Expression of miR-92a in the cells was measured by quantitative real-time PCR（qRT-PCR）.MTT assay was used to evaluate the cell proliferation.Cell apoptosis was detected by flow cytometry（AnnexinV-FITC/PI assay） and Hoechst 33258 staining.Results Expression of miR-92a in two cell lines was significantly down-regulated by the transfection of anti-miR-92a oligonucleotides for 48 h.At the same time,the cell proliferation was inhibited and apoptosis was increased remarkably by the transfection.Further,microscopic observation with Hoechst 33258 staining showed apoptotic feature of cell nucleus change in the transfected gastric cancer cell lines while there was no change in the control group.Conclusion Targeted inhibition of miR-92a with antisense oligoribonucleotide effectively suppresses gastric cancer cell growth by inducing apoptosis.miR-92a may be a potential target for gastric cancer therapy.
出处
《安徽医科大学学报》
CAS
北大核心
2012年第2期137-141,共5页
Acta Universitatis Medicinalis Anhui
基金
教育部高校博士学科点专项科研基金(编号:20100171120090)
广东省医学科研基金(编号:B2009079)
关键词
胃肿瘤
微小RNA
反义寡核苷酸
增殖
凋亡
stomach neoplasms
microRNAs
antisense oligonucleotides
proliferation
apoptosis
