摘要
目的:观察定心方对ApoE基因敲除小鼠(ApoE-/-小鼠)主动脉粥样硬化病变的影响,并初步探讨血清内脏脂肪素(visfatin)在其中的作用。方法:将40只8周龄ApoE-/-小鼠随机分为5组。模型组灌服等剂量生理盐水;定心方低、中、高剂量组灌服定心方药液9.295g/(kg.d)、18.59g/(kg.d)、37.18g/(kg.d);辛伐他汀组灌服辛伐他汀5mg/(kg.d)。另设相同基因背景和周龄的C57BL/6J小鼠为空白对照组。12周后测定小鼠体质量、血清生化指标及visfatin、hs-CRP、TNF-a水平;对主动脉粥样硬化病变进行病理学检查。结果:与模型组相比,定心方低、中、高剂量组小鼠体重增加值明显降低(P<0.05),定心方高剂量组血脂水平及血清visfatin、hs-CRP、TNF-α含量显著低于模型组(P<0.01)。定心方低、中、高组斑块面积分别较模型组减少39.3%、75.0%、86.5%(P<0.01)。结论:定心方可降低ApoE-/-小鼠血脂水平,抑制动脉粥样硬化的形成,其作用机制可能与降低血清visfatin水平有关。
Objective：To observe the effect of Dingxin Formula on visfatin in an apolipoprotein E knockout mouse model.Methods：forty male 8-week-old ApoE-/-mice were randomly divided into five equal groups：model group,low-dose Dingxin Formula [9.295g/（kg·d）] group,medium-dose Dingxin Formula [18.59g/（（kg·d）] group,high-dose Dingxin Formula [37.18g/（kg·d）] group,simvastatin [5mg/（kg·d）] group,Wildtype mice were used as normal control group（n=8）.After 12 weeks,the serum concentration of lipid,visfatin,hs-CRP and TNF-α were determined.Aorta were used for histomorphometric analysis by means of HE.Results：Compared with the model group,three dose Dingxin Formula groups of mice weight gain significantly lower（P0.05）.Dingxin Formula high-dose group of serum lipid and serum visfatin,hs-CRP,TNF-α content are significantly lower than the model group（P0.01）.Compared with model group,Dingxin Formula of low,medium,high,group reduced plaque size 39.3%,75.0%,86.5% respectively（P0.01）.Conclusion：Dingxin Formula can inhibit the development of atherosclerosis.The mechanism is related to modulation of visfatin.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2011年第6期79-82,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家自然科学基金资助项目(编号81072776)
