摘要
目的:研究IL-17对原代培养心肌细胞中单核细胞趋化蛋白-1(MCP-1)表达的影响。方法:差速贴壁法分离乳鼠心肌细胞进行原代培养,采用逆转录聚合酶链反应(RT-PCR)检测心肌细胞IL-17R和MCP-1基因表达情况,并采用酶联免疫吸附法(ELISA)检测心肌细胞培养上清中MCP-1蛋白的表达。结果:心肌细胞存在IL-17R的基因表达。IL-17呈浓度依赖式上调心肌细胞MCP-1基因和蛋白的表达水平,与不加刺激因子的对照组相比差异有统计学意义(P<0.05)。心肌细胞MCP-1 mRNA的表达量在IL-17作用4 h时达到高峰,随后开始下降,而IL-17作用下心肌细胞MCP-1蛋白的表达呈时间依赖式升高,与对照组相比有显著性差异(P<0.05)。结论:心肌细胞表达IL-17R。IL-17可上调心肌细胞MCP-1的表达,其作用与IL-17的浓度和作用时间有关。
AIM： To investigate the effect of interleukin-17（IL-17） on the expression of monocyte chemoattractant protein-1（MCP-1） in the primary cultured cardiac myocytes.METHODS： The cardiac myocytes were isolated from neonatal mice by different adhesion method.The expressions of IL-17R and MCP-1 in the cardiac myocytes were evaluated by reverse transcription-polymerase chain reaction（RT-PCR）.And the concentration of MCP-1 in the culture supernatant of the cardiac myocytes was detected by enzyme-linked immunosorbent assay（ELISA）.RESULTS： There was the expression of IL-17R in the cardiac myocytes.After stimulated by IL-17,the expressions of MCP-1 in the cardiac myocytes were significantly increased in dose-dependent manner compared with that of culture medium control（P0.05）.The amount of MCP-1 mRNA in the cardiac myocytes was the highest at 4 h after stimulated by IL-17.And then the amount of MCP-1 mRNA in the cardiac myocytes began to descend.The concentrations of MCP-1 in the culture supernatant of the cardiac myocytes increased in time-dependent manner and had significant differences with the control group（P0.05）.CONCLUSION： The cardiac myocytes could express IL-17R.IL-17 could up-regulate the expression of MCP-1 in the cardiac myocytes,which was associated with the dose and stimulation time of IL-17.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2012年第2期163-166,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
河南省科技攻关项目(0496060902)
