摘要
目的:检测新辅助化疗(NACT)后宫颈癌患者盆腔淋巴结内细胞凋亡,探讨新辅助化疗能否通过促进淋巴结内癌细胞凋亡控制宫颈癌淋巴结转移。方法:采用SP免疫组化技术和原位末端标记法(TUNEL)检测2002年2月至2008年10月间104例Ⅰb_2～Ⅱb期宫颈癌患者盆腔淋巴结的细胞凋亡情况,同时比较了其中54例宫颈原发癌灶的情况。结果:54例宫颈癌患者对新辅助化疗的总体反应率为74.07%(40/54),盆腔淋巴结转移率为20.37%(11/54)低于直接手术组的40.00%(20/50)。NACT组盆腔淋巴结和宫颈原发癌灶中Caspase-3的表达以及细胞凋亡率(AI)明显高于直接手术组(P<0.05);且盆腔淋巴结中细胞凋亡率(AI)与原发灶呈正相关(r=0.316,P=0.01)。结论:新辅助化疗通过诱导淋巴结内癌细胞凋亡从而控制宫颈癌盆腔淋巴结转移病灶。
Objective： To investigate the administration ofneoadjuvant chemotherapy （NACT） for controlling pelvic node metas- tasis by inducing tumor cell apoptosis in patients with cervical cancer. Methods： hnmunohistochemical SP staining and in situ terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （ TUNEL ） were employed to assay the pelvic lymph node apoptotic sta- tus in 104 patients with cervical cancer, i.e., 54 NACT cases with stage 1/3 and ⅡB cervical cancer awaiting surgery and 50 cases with stage IB and IA cervical cancer who have undergone immediate surgery. Among these cases, 54 （ 27 treated with NACT before surgery and 27 who underwent immediate surgery ） were assessed for the apoptotic status of the in situ diseased regions simultaneously. Re- suits： The total response rate of the chemotherapy to NACT was 74.07% in the 54 cases （ 40/54 ）, with a lower incidence of pelvic node involvement （ 11/54, 20.37% ） than the immediate surgery group （ 20/50, 40% ）. Both pelvic nodes and in situ diseased regions in the NACT group had a much higher caspase-3 expression and apoptotic index （ AI ） （ P 〈 0.05 ） than those in the control or immediate sur- gery group. Additionally, the AI value in the pelvic nodes was significantly correlated with that in the in situ lesions （ r = 0.316, P = 0.01 ）. Conclusion： NACT is efficient in controlling pelvic node metastasis by inducing tumor cell apoptosis in patients with regionally advanced cervical cancer.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2012年第3期141-144,共4页
Chinese Journal of Clinical Oncology
基金
教育部国际合作与交流司留学回国人员科研启动基金(编号:2007-1108)资助~~
关键词
宫颈癌
新辅助化疗
凋亡
淋巴结转移
Cervical cancer
Neoadjuvant chemotherapy
Apoptosis
Nodal metastasis
