摘要
目的从海绵中分离筛选具有抗H1N1活性的放线菌,并对活性菌株HA10201进行鉴定。方法采用CPE和MTT方法对从海绵中分离的放线菌进行抗H1N1活性筛选,对活性较强的菌株HA10201进行形态学和生理生化特性研究,测定其16SrDNA序列并进行系统发育分析。结果菌株HA10201发酵液稀释20倍后对H1N1抑制率达68.1%,HA10201与Streptomyces roseorubens的形态和生理生化特征最为接近,且与其16SrDNA序列相似性为99.40%,且在发育树上聚为一个分支。结论菌株HA10201鉴定为S.rose-orubens,其发酵液具有较强的体外抗H1N1活性,值得进一步研究。
Objective Screening of marine actinomycetes with anti- H1N1 virus activity from marine sponge and identification of the active strain HA10201. Methods Activity based on MTT assay and cyto- pathic effect （CPE） assay were carried out. The identification of the active strain HA10201 was deter- mined by morphological characteristics, physiological and biochemical tests and molecular phylogenetic analysis. Results The 20- fold diluted ferment filtrates of strain HA10201 had 68. 1~//00 anti- HIN1 virus suppression rate. Phylogenetic analysis based on 16S rRNA gene sequence showed that the strain HA10201 had a 99.40% homology with Streptomyces roseorubens. Conclusion Strain HA10201 was i- dentified as S. roseorubens. Its fermentation broth showed strong resistance to HIN1 virus. The active metabolites were worth to further study.
出处
《中国海洋药物》
CAS
CSCD
北大核心
2012年第1期6-9,共4页
Chinese Journal of Marine Drugs
基金
国家"863计划"(2007AA09Z447)
中央级公益性科研院所基金(1630012011004
ITBBZD2007-5-1)资助项目
关键词
放线菌
抗病毒
筛选
鉴定
actinomycete
anti-virus
screening
identification
