摘要
目的:分析新疆食管鳞状细胞癌和食管正常上皮细胞的差异表达蛋白.方法:运用激光捕获显微切割技术(LCM)分别获取食管鳞状癌细胞和食管正常上皮细胞,应用二维凝胶电泳技术(2-DE)分离纯化细胞,Imagemaster 2D软件比较分析两者电泳图谱的差异,基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)鉴定分析两者表达的差异蛋白.结果:建立了食管鳞状癌细胞和食管正常上皮细胞的2-DE图谱,获得43个差异蛋白点,通过质谱鉴定出17种蛋白,其中15种蛋白如Trangelin2、HSP27、S100A11、GSTP等在食管鳞状癌细胞中表达明显增高,2种蛋白如SCCA1,在食管鳞状癌细胞中表达明显降低.结论:提示17种差异蛋白可能与食管鳞状细胞癌的发生和发展有关,为筛选食管鳞状细胞癌的特异性分子标志物奠定基础.
AIM：To analyze differentially expressed proteins between human normal esophageal epithelial cells （NEEC） and esophageal squamous cell carcinoma （ESCC） cells.METHODS：NEEC and ESCC cells were cut by laser capture microdissection （LCM）,and total proteins of the cells were separated by twodimensional gel electrophoresis （2-DE）.2-DE images were analyzed using Imagemaster 2D software.Differential proteins between NEEC and ESCC cells were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry （MALDI-TOF-MS）.RESULTS：A total of 43 protein spots were discovered and there were 17 differentially expressed proteins between NECC and ESCC.Of them,15 （such as Trangelin2,HSP27,S100A11 and GSTP） were up-regulated and two （such as SCCA1） down-regulated in ESCC.CONCLUSION：The differentially expressed proteins identified in this study may play a role in the carcinogenesis and development of ESCC and represent potential molecular biomarkers for ESCC.
出处
《世界华人消化杂志》
CAS
北大核心
2011年第36期3682-3686,共5页
World Chinese Journal of Digestology
基金
新疆地方病分子生物学实验室开放基金资助项目
No.XJDX0208-2006-09~~
关键词
食管鳞状细胞癌
蛋白组学
差异表达蛋白
二维凝胶电泳
基质辅助激光解吸电离飞行时间质谱
Esophageal squamous cell carcinoma
Proteomics
Differential proteins
Two-dimensional gel electrophoresis
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry
