摘要
目的探讨三聚氰胺（melamine，Mel）诱导大鼠肾脏损伤的机制。方法雄性SD大鼠48只随机分为4组，每组12只。A组为空白对照组，喂标准颗粒饲料和饮用自来水；B组为结石诱导组，喂含3％Mel的颗粒饲料和饮用自来水；C组喂食含3％Mel的颗粒饲料和饮用含2％牛磺酸的水；D组喂标准颗粒饲料和饮用含2％牛磺酸的水。共喂养3个月。每周收集大鼠24h尿液检测pH、肌酐、尿酸、蛋白、8-IP、H2O2和Mel水平。第3个月末处死全部大鼠，采血检测肌酐，进行肾脏病理分析（HE和OilRedO染色）及线粒体分离和检测。结果A组和D组尿液中未检测出Mel，B组和C组第1—4周尿液中Mel浓度分别为（3．16±0．45）、（4．39±0．21）、（5．40±0．28）、（5．50±3．26）mg／ml和（3．52±0．49）、（4．32±0．13）、（5．34±0．40）、（5．46±2．99）mg／ml，与A组比较，B组和C尿液Mel浓度有药物暴露时间依赖性。A组尿蛋白、尿肌酐、肌酐清除率、肾脏／体质量比值分别为（6．45±1．45）mg／24h、（28．0±7．4）mmol／L、（0．56±0．03）ml·min^-1·100g^-1、（2．29±0．89）mg／g，B组和C组分别为（14．56±7．69）ms／24h、（56．8±5．2）mmoL／L、（0．29±0．05）ml·min^-10g^-1．16±0．27）mg／g和（16．44±6．29）ms／24h、（55．8±7．4）mmol／L、（0．30±0．07）ml·min^-1·100g^-1、（4．40±0．56）mg／g，与A组比较，B组和C组尿蛋白显著增多，尿肌酐减少，肌酐清除率降低，肾脏／体质量比值增加。与B组比较，C组肾功能无明显变化，血清肌酐，尿蛋白降低不明显，差异无统计学意义（P〉0．05）。B组和C尿H228-IP及线粒体氧化检测试剂SOD、GSH-PX值分别为（28．5±5．2）mmol／L、（3．26±1．6）ps／ml、（21．1±7．8）U／mgprot、（19．0±2．5）活力单位和（26．7±4．8）mmol／L、（2．99±8．5）pg／ml、（20．3±6．9）U／mgprot、（
Objective To investigate the mechanism of melamine-induced renal damage in rats. Methods 48 male SD rats were randomly divided into 4 groups with 12 in each group and feed for 3 months. Group A were the control group, feed with standard granule feedstnff and drinking tap water. Group B were stone-induced group, feed with granule feedstuff containing 3% Mel and drinking tap water. Group C were feed with granule feedstuff containing 3% Mel and drinking water containing 2% taurine. Group D were feed with standard granule feedstuff and drinking water containing 2% taurine. Every week 24 h urine was collected to test PH, SCr, uric acid, protein, 8-IP, H2O2 and Mel level. All rats were sacrificed at the end of 3 months. Blood creatinine detection, renal pathology analysis （ HE and Oil eprred 0 dyeing, immunohis- tochemieal） and mitoehondria separation and detection were undertaken. Results Mel was not detedted in urine of Group A and Group D. The urine concentration of Mel in Group B and Group C in 1 week, 2 weeks, 3 weeks, 4 weeks were 3.16 ±0.45, 4.39 ±0.213, 5.40 ±0.28, 5.50 ±3.26 and 3.52 ±0.49, 4.32±0. 135, 5.34 ± 0.40, 5.46 ± 2.99 mg/ml, respectively. Compared with Group A, the Mel concen tration in urine of Group B and C were drug exposure time dependent. In Group A, the urine protein, urine creatinine clearance, serum creatinine, and renal/weight ratio were 6.45± 1.45 mg/24 h, 28.0±7.4 mmol/1, 0.56±0.03 ml · min^-1 · 100g^-1, 2.29 ±0.89 mg/g, while in Group B and C, the urinary protein urine, serum creatinine, creatinine clearance, kidney/weight ratio were 14.56 ±7.69, 56.8 ±5.2, 0.29 ±0.05, 4.16 ±0.27 and 16.44 ±6.29, 55.8 ±7.4, 0.30 ±0.07, 4.40 ±0.56, respectively. Compared with group A, in Group B and C, the urinary protein increased significantly, urine creatinine clear ance reduced, serum creatinine reduced, and renal/weight ratio increased. Compared with Group B, the improvement of renal function in Group C was not significant , and the decrease of serum creatinine and urinary pr
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2012年第1期32-36,共5页
Chinese Journal of Urology
基金
广西科学研究与技术开发计划课题（桂科攻0897006）
