摘要
目的:研究由人脐带间充质干细胞(UCMSCs)分化而来的汗腺样细胞在冻存与复苏后的生物学活性与促汗腺再生能力。方法:分离培养人UCMSCs和人汗腺细胞,通过两种细胞共培养方式促进UCMSCs向汗腺细胞分化。分化后的干细胞经冻存、复苏处理后,局部移植于裸鼠烫伤脚掌,通过组织学观察和发汗试验观察汗腺组织再生修复情况。结果:由UCMSCs分化而来的汗腺样细胞经冻存、复苏、移植于裸鼠脚掌创面后,能够在创面形成汗腺结构并具有发汗功能。结论:UCMSCs经诱导分化为汗腺样细胞后,经冻存和复苏处理,在体移植后仍能够有效促进受损汗腺的修复与再生,有望为大面积重度烧伤患者汗腺的修复提供充足的种子细胞。
Objective： To explore the biological characteristics and the function of sweat gland-like cells transdifferentiated from umbilical cord mesenchymal stem cells （UCMSCs） after being deep freeze storage. Methods： Human UCMSCs and sweat gland cells were isolated, cultured and identified with specific markers. They were cocultured to transform the UCMSCs to sweat gland-like cells. After being deep frozen and defrosted, the stem cells, which exhibited the phenotype of sweat gland cells, were implanted into scald injured paws of nude mice. The regeneration of the sweat gland cells was observed. Results： The transdifferentiated sweat gland-like cells were successfully implanted into the scald paws of nude mice after being frozen and defrosted, which regenerated the function of perspiration. Pathological observation showed that sweat glands were regenerated. Conelusions：UCMSCs can be transdifferentiated into sweat gland-like cells in vitro, and the deep frozen and defrosted sweat gland like ceils can be implanted into a fresh wound to regenerate sweat glands.
出处
《感染．炎症．修复》
2011年第4期202-204,F0002,共4页
Infection Inflammation Repair
基金
国家973计划资助项目(2012CB518105)
国家自然科学基金(81121004
81000843)
关键词
脐带间充质干细胞
汗腺
再生
Umbilical cord mesenchymal stem cells Sweat glands Regeneration
