摘要
[目的]探究呋喃丹和氯氰菊酯的类雌激素活性。[方法]采用重组人雌激素受体α基因的双杂交酵母测定了17β-雌二醇和2种农药诱导产生β-半乳糖苷酶活性的EC50值。[结果]17β-雌二醇、呋喃丹、氯氰菊酯对应的EC50值分别为4.04×10-10、2.34×10-7、1.04×10-6mol/L,诱导的最大酶活分别为4.97、1.82、1.59 U。[结论]2种农药均具有不同程度的类雌激素活性,且呋喃丹的类雌激素活性高于氯氰菊酯。
the aim is to explore the estrogenicity of carbofuran and cypermethrin.[Methods] The EC50 values of the activity of β-galactosidase induced by 17β-estradiol,carbofuran and cypermethrin by recombining human estrogen receptor α gene yeast was determined.[Results] The EC50 values of 17β-estradiol,carbofuran and cypermethrin are 4.04×10-10,2.34×10-7,1.04×10-6 mol/L respectively and the maximum enzyme activities are 4.97,1.82 and 1.59 U respectively.[Conclusions] Carbofuran and cypermethrin have different levels of estrogenicity while carbofuran is shown to have higher estrogenicity.
出处
《农药》
CAS
北大核心
2012年第2期105-107,150,共4页
Agrochemicals
基金
国家自然科学基金(30771429
31071653)
教育部留学归国人员科研基金
