摘要
[方法]通过51 dph(days post hatch)稀有鮈鲫幼鱼在不同质量浓度17β-雌二醇(E2)中经过28 d的流水式暴露研究,观察了E2对稀有鮈鲫幼鱼生长状态、卵黄蛋白原(VTG)含量、性别比率和性腺组织学的影响。[结果]观察发现,与对照组相比,259、1180 ng/LE2处理组中的稀有鮈鲫幼鱼体长生长受到显著性抑制(P≤0.05,P≤0.01);8、48、259 ng/L处理组雌鱼VTG含量水平显著降低(P≤0.01),而雄鱼在1180 ng/L处理组中V T G含量水平显著升高(P≤0.01);通过组织病理学观察,E2对稀有鮈鲫幼鱼雌雄性别比率未产生显著影响,但在48 ng/L处理组出现雌雄间体现象,同时,在259、1180 ng/L处理组中,雄鱼精巢出现明显的萎缩。[结论]17β-雌二醇对51 dph的稀有鮈鲫性腺分化产生了雌激素效应,但用稀有鮈鲫作为筛选和评价内分泌干扰物的活体试验模型还需要进一步研究。
[Methods] In present study, 51 days post hatch （dph） juveniles of rare minnows （Gobiocypris rams） were exposed to various concentrations of 17β-estradiol for 28 days in flow-through conditions. Growth performance, vitellogenin, sex ratio and gonadal histopathology were analyzed. [Results] Compared with control, the results showed that the length increase was inhibited at 259 and 1180 ng/L （P≤0.05, P≤0.01）. And VTG concentrations in female fishes were decreased significantly at 8, 48 and 259 ng/L E2 treated groups （P≤0.01）, and in male fishes were increased significantly at 1180 ng/L E： treated group （P≤0.01） compared with control. Based on histopathologic examination, no obviously difference in sex ratio was found between the treatment and control groups, however, intersex was found at 48 ng/L E2 treatment group, and at 259, 1180 ng/L E： treatment groups, male fishes spermaries appeared visibly depauperate. [Conclusions] 17fl-Estradiol performed estrogenic effects on gonads differentiation of the 51 dph juveniles of rare minnows, and it need to be studied further to take rare minnows as survival experimental prototype to screen and evaluate endocrine disrupters.
出处
《农药》
CAS
北大核心
2012年第7期513-516,共4页
Agrochemicals
基金
国家科技支撑计划课题"农药安全性
生态评价研究"(2011BAE06B09)
