摘要
目的观察靶向缺氧诱导因子2(HIF-2)的小干扰RNA(siRNA)对人胰腺癌BXPC-3细胞增殖和凋亡的影响。方法将BXPC-3细胞随机分为A、B、C组,A组转染HIF-2 siRNA、B组转染非特异性siRNA、C组不转染,分别予常氧、缺氧环境下培养;采用MTT法检测培养24、48、72、96 h时BXPC-3细胞的光密度(OD)值观察增殖情况,用流式细胞仪检测培养48 h时BXPC-3细胞凋亡率。结果在缺氧培养48 h时,A、B、C组细胞OD值分别为0.54±0.07、0.69±0.09、0.72±0.13,72 h时分别为0.55±0.11、0.88±0.07、0.88±0.05,96 h时分别为0.56±0.12、0.93±0.11、0.94±0.09;A组与B、C组比较,P均<0.05。在缺氧培养48 h时,A、B、C组细胞凋亡率分别为21.37%±0.17%、7.12%±0.03%、7.24%±0.07%;A组与B、C组比较,P均<0.05。在常氧状态下,各组细胞OD值及凋亡率无明显差别。结论缺氧状态下,靶向HIF-2 siRNA可抑制人胰腺癌BXPC-3细胞增殖,诱导其凋亡。
Objective To observe the effect of hypoxia-inducible factor 2(HIF-2) small interference RNA(siRNA) on the proliferation and apoptosis in human pancreatic carcinoma BXPC-3 cells. Methods BXPC-3 cells were divided into group A, B and C randomly. Cells in group A were transfected with HIF-2 siRNA, in group B with nonspecific siRNA and " group C uninfected. Cells in each group were cultured with normoxic environment and hypoxic state. The cell proliferation was assessed by MTT assay at 24, 48, 72, 96 h and the changes in cell apoptosis were evaluated by flow cytometry at 48 h. Results At the 48 h of hypoxic state, the OD value of group A, B and C were respecti.vely 0.54 ± 0.07, 0.69 ± 0.09, 0.72 ±0.13, at the 72 h are respectively 0.55 ±0. 11, 0.88 ±0.07, 0.88 ±0.05, at 96 h were respectively 0.56 ± 0.12, 0.93 ±0.11,0.94 ±0.09. Compared with group B and C, cell proliferation in group A was inhibited remarkably ( all P 〈 0.05). At the 48 h of hypoxic state culture, the apoptosis rate of group A, B and C were respectively 21.37% ± 0.17% , 7.12% ±0.03%, 7.24% ±0.07%. Compared with group B and C, the apoptosis in group A was increased sig-nificantly ( all P 〈 0.05 ). In normoxic environment, there was no significant difference between the OD value and apoptosis rates of each group. Conlusion In hypoxic environment, HIF-2 siRNA can inhibit cell proliferation, promote apoptosis in human pancreatic carcinoma ceils.
出处
《山东医药》
CAS
2012年第2期18-20,共3页
Shandong Medical Journal
基金
中国博士后科学基金资助项目(2011M500697)
山东省博士后创新项目专项基金资助(201003048)
关键词
RNA干扰技术
缺氧诱导因子
细胞增殖
细胞凋亡
胰腺癌细胞
胰腺肿瘤
RNA interference
hypoxia-inducible factor
cell proliferation
cell apoptosis
pancreatic cancer cell pancreatic neoplasms
pancreatic carcinoma