摘要
目的研究不同时间点旋毛虫肠道急性感染后外周血及肠道黏膜内调节T细胞(CD 4+/CD 25+Treg)的变化规律。方法研究分为旋毛虫急性感染组(感染后2周,4周及8周)、对照组(与感染组相应年龄及饲养环境)。测定肠道病理损伤程度,外周血中及肠道黏膜固有层淋巴细胞中CD 4+/CD 25+T细胞占CD 4+淋巴细胞的百分比。结果 (1)模型动物感染后2周肠道各部位炎症评分明显高于正常组(回肠末端2.85±0.22,近端结肠2.66±0.27,远端结肠2.43±0.19,P<0.05);4周组炎症评分较2周组降低,但仍高于正常组;至感染后8周大致恢复正常。(2)感染2周后外周血Treg百分比明显下降(3.91±0.15 vs 2.28±0.13,P<0.01),下降比例41.7%。造模后4周,外周血Treg百分比仍低于正常对照组(3.91±0.15 vs 3.01±0.14,P<0.01)。而至模型诱导成功的8周后,血Treg的比例(CD 4+CD 25+/CD 4+)趋于正常(3,48±0.15,0.01<P<0.05)。(3)在急性旋毛虫感染2周后LPL-Treg百分比明显下降(8.32±0.70 vs 3.2±0.29,P<0.01),下降比例60.4%。造模后4周,LPL-Treg百分比仍低于正常对照组(8.32±0.7 vs 4.67±0.36,P<0.01)。而至模型诱导成功的8周后,LPL-Treg的比例(CD 4+CD 25+/CD 4+)大致恢复正常(7.86±0.38,P>0,05)。结论旋毛虫急性感染诱导感染后肠易激综合征模型中,调节T细胞水平下降。Treg的参与可能与PI-IBS的发生有关。
Objective To investigate the change of blood Tregulatory cell（Treg）after different time point of acute trichinella infection.Methods Male sprague-dawley rats were divided 4 groups in terms of sampling time：control group and PI-IBS group（2 weeks,4 weeks and 8 weeks after acute trichinella infection）.Flow cytometry was used to detect the percent of Treg both in blood and laminal propria lymphocyte（LPL）.Results（1）The intestinal pathologic scores in 2 and 4 weeks groups are higher than that in control group;the scores in 8,12 weeks groups recover from the intestinal inflammation.（2）2 weeks after trichinella spirais infection,there is a significant decrease of CD 4＋ CD 25＋/CD 4＋both in peripheral blood and LPL,which is almost abolished in 8 weeks group.Conclusion There is declination of blood Treg and LPL-Treg percent after the early stage of acute trichinella infection,and this might involved in the pathogenesis of post-infectioas irritable bowel syndrome,
出处
《临床消化病杂志》
2011年第6期349-352,共4页
Chinese Journal of Clinical Gastroenterology
