摘要
目的观察金纳多预处理后，水通道蛋白-1（AQP-1）在大鼠离体肺缺血再灌注损伤过程中的表达及其功能。方法将40只大鼠随机分为供体组和受体组（rt=20）。供体组再随机分为2组（n=lo），低钾右旋糖苷液（LPD）灌注组与LPD＋金纳多组；受体组也随机分为2组（n=10）。LPD灌注组大鼠左肺设为A组，右肺设为B组，为对照组。LPD＋金纳多灌注组左肺设为C组，右肺设为D组，为对照组。建立大鼠离体肺缺血再灌注模型，分组造模后，取肺组织，免疫组织化学法检测肺组织AQP-1的表达；RT-PCR检测肺组织AQP-1mRNA的表达；免疫印迹法检测肺AQP-1蛋白的表达。结果A组及c组肺组织中AQP-1蛋白表达的相对灰度值分别为0．65±0．06、0．88±0．11，两者比较差异有统计学意义（P〈0．05）。AQP．1在A组及C组肺组织中的mRNA表达平均相对A值分别为0．30．4-0．08、0．49±0．11，两者比较差异有统计学意义（P〈0．05）。结论肺移植术前给予金纳多治疗，可减轻供肺的损伤。
Objective To explore the expression and function of aquaporin-1 （ AQP-1 ） in rat lung with ischemia/reperfusion （I/R） injury after pretreatment with Ginaton. Methods Adult Wistar rats were divided into 2 groups. Twenty rats served as donors （ n = 20）, and 20 as recipiems （ n = 20）. Left lungs of donor rats were used as isolated lung reperfusion model and perfused only with LPD solution as group A （n = 10）. The rats pretreated with Ginaton before reperfusion served as group C （n = 10）. Right lungs of donor rats without any treatment served as control group （ group B and group D, n = 10 each）. After the model was established, the expression of AQP-1 in the lung tissues was detected by using immunohisto- chemistry, Western blotting, and reverse transcriptase-polymerase chain reaction. Results Immunohisto- chemical examination revealed that AQP-1 was expressed in endothelia. Immunoblotting studies demonstra- ted that AQP-1 protein in groups A ant C was 0. 65 ＋ 0. 06 and 0. 88 ＋ 0. 11 respectively. The mRNA ex- pression in groups A and was 0. 30 ＋0. 08 and 0. 49 ＋0. 11 respectively. The expression of AQP-1 protein and mRNA in group C was significantly higher than in group A （ P 〈 0. 05 ）. Conclusion The pretreatment with Ginaton can reduce the acute lung injury caused by I/R injury.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2012年第2期267-269,共3页
Chinese Journal of Experimental Surgery
基金
河南省卫生厅医学科技攻关计划资助项目（2011020029）
