摘要
目的观察脾切除对大鼠肝大部分切除术后细菌移位的影响。方法将SD大鼠随机分为3组：假手术组（SO组），2／3肝切除组（PHx组），肝切除加脾切除组（PHx＋sp组）。85只大鼠中30只用于测定门静脉压力，55只用于分析细菌移位、血浆内毒素、血浆D-乳酸、肠组织病理学及免疫功能。结果PHx组门静脉压力（13．34±0．64）cmH2O（1cmH：0=0．098kPa）显著高于SO组（7．64±0．44）cmH：0和PHx＋Sp组（10．30±0．69）cmH：0（P〈0．01）。PHx组的细菌移位率高于SO组（65．00％比6．67％；P〈0．01），也明显高于PHX＋sp组（25．00％；P〈0．05）。大鼠血浆内毒素中位数SO组为0．00ng／L，PHx组为4．05ng／L，PHx＋sp组为1．47ng／L（P〈0．01）。血浆D．乳酸在s0组和PHx＋Sp组也较PHX组低（分别为1．68、23．36、39．09g／L，P〈0．01）。结论脾切除能降低肝大部分切除大鼠门静脉压力，增强肠黏膜屏障功能，减少内毒素和细菌移位的发生。
Objective To investigate the effects of splenectomy on bacterial tranlocation after ma- jor liver resection. Methods Rats were divided into three groups ： the sham operation group （ SO group）, the two-thirds partial hepatectomy group （PHx group） and the two-thirds partial hepatectomy plus splenec- tomy group （PHx ＋ Sp group）. Portal pressure was measured in 30 rats, and BT, endotoxemia, intestinal permeability to D-lactic acid, intestinal histology and immune function were analyzed in 55 rats. Results Portal pressure in the PHx rats [ （ 13. 34 ＋0.64） cm H20 （ 1 cm H20 =0. 098 kPa） ,P 〈0. 01 ] was sig- nificantly higher than in the SO rats （7.64~ 0.44） emH20 and the PHx ＋ Sp rats （10.30~ 0. 69） em H20. In addition, the rate of BT was higher in the PHx rats than in the SO rats （65.00% vs 6. 67% ;P 〈0. 01 ）. Splenectomy significantly reduced BT in the PHx rats （25.00% ;P 〈0. 05）. Endotox- emia was not evident in the SO rats （0. 00 ng/L） and blood endotoxin levels were decreased in the PHx ＋ Sp rats （1.47 ng/L） as compared with the PHx rats （4. 05 ng/L,P 〈0. 01 ）. D-lactic acid level was also higher in both the PHx and PHx ＋ Sp rats than in the SO rats （39. 09, 23.36, and 1.68 g/L respectively; P 〈 0. 01 ）. Conclusion Splenectomy induced improvement in the intestinal histology, lowered portal pres- sure, enhanced intestinal barrier function and diminished blood endotoxin levels and BT in rats with major liver resection. Spleneetomy might be useful in the prevention of infection after major liver resection.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2012年第2期240-242,共3页
Chinese Journal of Experimental Surgery
基金
广州市科技计划资助项目
关键词
肝部分切除术
脾切除
细菌移位
肠屏障
内毒素
Partial hepatectomy
Splenectomy
Bacterial tranlocation
Gut barrier
Endo-toxin
