摘要
目的探讨ω-3多不饱和脂肪酸（ω-3PuFA）对创伤性休克大鼠急性肺损伤的影响。方法雄性Wistar大鼠36只，体重240～260g，月龄3月，采用随机数字表法，将大鼠随机分为3组（n=12）：对照组（c组）；创伤性休克组（佟组）采用骨折复合失血致创伤性休克；ω-3PUFA组于造模前12h及2h经尾静脉注射ω-3PUFA2ml／kg，C组和鸭组注射生理盐水2ml／kg。模型制备成功后120min时采集颈动脉血样，处死取肺组织。用ELJSA法检测血清TNF．a、8．异前列腺素F2-4（8-iso—PGF2。）、IL-1β及IL-10的浓度；计算肺组织湿/干重比（W／D比）；光镜下观察肺组织病理变化，行病理学评分。结果与c组比较，髑组和ω-3PuFA组血清TNF-α、8-iso-PGF2a、IL-1β及IL10的浓度、肺组织w／D比及病理学评分明显升高（P〈0．01）。与TS组比较，ω-3PUFA组血清TNF-α、8-iso—PGF2a及IL-1β的浓度、肺组织W／D比及病理学评分明显降低，血清IL-10浓度升高（P〈0．05或0．01）。结论60．3PUFA可有效地抑制创伤性休克大鼠全身炎性反应，减轻急性肺损伤。
Objective To investigate the effects of ω-3 polyunsaturated fatty acid （ω-3 PUFA） on acute lung injury （ALI） in a rat model of traumatic shock. Methods Thirty-six male Wistar rats aged 3 months were randomly assigned into 3 groups （n = 12 each）： control group （group C）; traumatic shock group （group TS） and ω-3 PUFA ＋ TS group （group ω-3 PUFA）. Traumatic shock was induced by fracture of femur and hemorrhage according to the method described by Feeney in groups TS and ω-3 PUFA. In group ω-3 PUFA, ω-3 PUFA 2 ml/kg was injected via the caudal vein at 12 and 2 h before induction of traumatic shock. Arterial blood samples were taken at 120 min after traumatic shock was successfully induced for determination of serum concentrations of TNF-α, IL-1β, IL-10 and 8-iso-PGF2α by ELISA. The animals were then sacrificed and lungs were removed for determination of W/D lung weight ratio and microscopic examination. Results Traumatic shock significantly increased serum concentrations of TNF-α, IL-1β, IL-10 and 8-iso-PGF2a, W/D ratio and pathologic scores of lung tissues in groups TS and ω-3 PUFA as compared with group C. ω-3 PUFA significantly attenuated traumatic shock-induced increase in serum concentrations of TNF-α, IL-1β and 8-iso-PGF20, W/D ratio and pathologic scores of lung tissues but further increased the serum IL-10 concentration in group ω-3 PUFA as compared with group TS. Conclusion ω-3 PUFA can significandy inhibit the systemic inflammatou response and ameliorate traumatic shock-induced ALI.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2011年第5期624-626,共3页
Chinese Journal of Anesthesiology
