摘要
目的 观察亚砷酸钠（NaAsO2）诱导人膀胱上皮永生化细胞（SV-HUC-1）氧化损伤作用.方法 以不同浓度NaAsO2[0（对照）、1、2、4、8、10μmol/L]对SV-HUC-1细胞染砷24 h,利用流式细胞仪检测细胞内活性氧（ROS）水平,采用酶联免疫吸附实验（EHSA法）检测细胞内硝基酪氨酸（NT）含量和细胞培养液中8-羟基脱氧鸟嘌呤核苷（8-OHdG）水平.结果 1、2、4、8、10 μmol/L染砷组SV-HUC-1细胞ROS水平（81.76±4.91、95.23±2.17、126.61±17.95、126.74±27.77、114.18±9.65）明显高于对照组（69.84±1.28,P＜ 0.05或＜ 0.01）,ROS水平与染砷剂量呈显著正相关（r=0.818,P＜ 0.01）.10 μmol/L染砷组SV-HUC-1细胞内NT含量[ （919.66±206.33）μg/L]显著高于对照组[（238.19±38.28） μg/L,P＜ 0.01],NT含量与染砷剂量呈显著正相关（r=0.617,P＜0.01）.各组细胞培养液中8-OHdG含量比较,差异无统计学意义（F=2.127,P＞0.05）.结论 NaAsO2能够引起SV-HUC-1细胞氧化损伤.
Objective To study the state of oxidative injury induced by sodium arsenite（NaAsO2） in SV-40-immortalized normal uroepithelial （SV-HUC-1 ） cells.Methods SV-HUC-1 cells were exposed to different concentrations of NaAsO2[0（control）,1,2,4,8,10 μmol/L] for 24 h,intracellular reactive oxygen species （ROS） was determined by flow cytometry,and the content ofintracellular nitrotyrosine（NT） and the 8-Hydroxydeoxyguanosine （8-OHdG） levels of cell culture medium were detected by enzyme linked immunosorbent assay （ELISA）.Results After 24 h treatment,ROS levels（81.76 ± 4.91,95.23 ± 2.17,126.61 ± 17.95,126.74 ± 27.77,114.18 ± 9.65） of SV-HUC-1 cells in the 1,2,4,8,10 μmol/L NaAsO2 exposure groups were significantly higher than those of the control group （69.84 ± 1.28,P 〈 0.05 or 〈 0.01 ）,ROS levels and exposure dose were positively correlated significantly（r =0.818,P〈 0.01）; the content of NT in the 10 μmol/L NaAsO2 exposure group[（919.66 ± 206.33） μg/L] was significantly higher than that in the control group[ （238.19 ± 38.28）μg/L,P 〈 0.01 ],NT content and dye concentrations of arsenic also had dose-response relationship （r =0.617,P 〈 0.01）; after 24 h the cells were treated with arsenic,no significant difference of 8-OHdG content in the culture medium was observed（F =2.127,P 〉 0.05 ）.Conclusion NaAsO2 can cause SV-HUC-1 cell oxidative damage.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2012年第1期13-15,共3页
Chinese Jouranl of Endemiology
基金
国家自然科学基金（30771865、81072244）
关键词
砷中毒
亚砷酸盐类
上皮细胞
膀胱
创伤和损伤
氧化还原
Arsenic poisoning
Arsenites
Epithelial cells, urinary bladder
Wounds and injuries, oxidation-reduction
