摘要
目的牛磺酸干预对亚慢性锰中毒大鼠基底前脑胆碱能神经细胞的影响。方法 SPF级雄性SD大鼠随机分为生理盐水对照组、染锰Ⅰ组、牛磺酸预防组、染锰Ⅱ组、牛磺酸治疗组。各组大鼠染毒结束后进行水迷宫实验检测大鼠空间学习记忆能力的变化,免疫组化染色观察胆碱乙酰转移酶(Choline acetyltransferase,ChaT)阳性神经元形态及数量变化。结果牛磺酸预防组平均逃避潜伏时间较染锰Ⅰ组显著缩短(P<0.05),牛磺酸治疗组与染锰Ⅱ组相比显著缩短(P<0.05),牛磺酸预防组平均平台搜索次数较染锰Ⅰ组增多(P<0.05),牛磺酸治疗组与染锰Ⅱ组相比差异无统计学意义(P>0.05)。染锰Ⅰ组基底节斜角带垂直支臂核(vDB)/水平支臂核(hDB)ChaT阳性细胞数明显少于对照组(P<0.05)。牛磺酸预防组hDB ChaT阳性细胞数较染锰组显著增多(P<0.05)。牛磺酸治疗组vDB ChaT阳性细胞数显著少于染锰Ⅱ组(P<0.05),而hDB ChaT阳性细胞数与染锰Ⅱ组无差异。结论牛磺酸预防或治疗改善染锰所致的学习记忆障碍,染锰致基底前脑ChaT阳性细胞显著减少,牛磺酸预防明显增加基底前脑hDB ChaT阳性细胞数量,而牛磺酸治疗组并未观察到胆碱能神经细胞增加。
Objective To study the effect of taurine on basal forebrain choline acetyhransferase positive cells of rats induced by MnC12·4H20. Methods SPF SD rats were randomly assigned into 5 groups： Control, MnC12-treated Ⅰ group, MnC12-treated Ⅱ group, Taurine-prevented group and Taurine-treated group respectively. Water Morris Maze is utilized to probe rats learning and memory capacity after 8 weeks injection. Morphological change and number of ChaT positive neurons from basal forebrain were observed under Nikon microscope after immunohistochemistry. Results The average escapes latencies in both taurine-prevented and taurine-treated are evidently shorter than those in MnCl2-treated（P 〈0.05）. The probing times in taurine-prevented is obviously increase than that in MnCl2-treated Ⅰ group （ P 〈 0. 05 ）. There is no significance defference between taurine-treated and MnCl2-treated Ⅱgroup. The ChaT positive neurons number of basal forebrain vDB/hDB in MnCl2-treated group is less than that in control group（P 〈 0.05）. Taurine-prevented reverse distinctly hDB ChaT number induced by MnCl2 （P 〈 0.05）. The vDB ChaT positive neurons number in taurine-treated is markedly less than that in MnCl2- treated Ⅱ group（ P 〈 0.05 ）. Conclusion Taurine-prevented and taurine-treated can improve impaired memory and study capacity induced by Sub-chronic MnC12 administration. Taurine-prevented may reverse markedly decreased hDB ChaT positive neurons of basal forebrain induced by MnC12. However, It can＇t be detected in taurine-treated group.
出处
《毒理学杂志》
CAS
CSCD
北大核心
2011年第6期399-402,共4页
Journal of Toxicology
基金
国家自然科学基金课题(30660156)
广西研究生教育创新计划(2009105981004D24)
关键词
牛磺酸
锰
学习记忆
胆碱乙酰转移酶
Taurine
Manganese
Memory and study
Chorine acetyltransferase
