摘要
目的探讨蛹虫草提取物(RW)对结肠癌细胞株SW111C的抑制作用及可能的机制。方法通过四甲基耦氮唑盐(MTT)法、台盼蓝拒染法检测RW对SW111C增殖的抑制作用,琼脂糖(DNA)凝胶电泳检测细胞凋亡;流式细胞术(FCM)PI染色法检测RW所引起SW111C的凋亡百分率和细胞周期的分布变化。结果 MTT法与台盼蓝拒染法检测结果表明RW对SW111C细胞有明显抑制作用,RW作用24、48、72h后的IC50分别为77.78、32.19、26.20mg/L,且这种抑制作用呈现浓度和时间的依赖性;DNA凝胶电泳检测发现32mg/L RW组出现DNA"梯状"条带;FCM检测发现凋亡率随浓度的增高而增高,2、8、32mg/L的凋亡率分别为(6.8±2.32)%、(11.8±2.58)%、(16.9±1.93)%,与对照组[(1.9±1.3)%]相比差异有统计学意义(P<0.01)。细胞周期阻滞于G1期,S期细胞减少。结论 (1)RW对体外培养的SW111C细胞的增殖有抑制作用,并呈现浓度和时间的依赖性,这种抑制作用可能与诱导SW111C细胞凋亡、改变其细胞周期分布有关。
Objective To investigate the proliferation effect of human colon cell line SW111C by extract from coredyceps militaris in vitro and to explore its anti-tumor mechanism from two factors：apoptosis,cell cycle.Methods The influences of extract from coredyceps militaris on cell growth inhibition were examined by MTT assay and trypan blue dying;Apoptosis was detected by flow cytometry,the changes of cell cycle were detected by FCM.Results The growth inhibition of SW111C treated by various concentration of extract from coredyceps militaris after different time was observed.The IC50 for SW111C cell were 77.78,32.19,26.20 mg/L after 24,48,72 h incubation respectively.The results showed that the growth inhibition presented dose and time dependent.The extract can induce SW111C cell apoptosis and block the cell cycle at G1 phase treated for 72 hours.Conclusion The extract from coredyceps militaris effectively inhibits the growth of human colon cancer cell lines SW111C in a dose and time dependent.The growth inhibitory effect of the extract on SW111C cells are involved in induction of cell apoptosis,block of the cell cycle and down-regulation of the telomerase activity.
出处
《重庆医学》
CAS
CSCD
北大核心
2012年第1期61-63,共3页
Chongqing medicine
关键词
结肠肿瘤
蛹虫草
细胞凋亡
细胞周期
colonic neoplasms
coredyceps militaris
apoptosis
cell cycle
