摘要
目的分析正常乳腺组织和浸润性导管癌中乙酰肝素酶(HPA)和GA结合蛋白α(GABPα)的表达及临床意义,探讨其在乳腺癌发生、发展中的作用及相关性。方法免疫组织化学方法检测HPA及GABPα的表达,分析二者的相关性及与临床病理因素的关系;Western blot检测HPA和GABPα蛋白的表达。结果免疫组化显示HPA和GABPα在浸润性导管癌中阳性表达率明显高于正常乳腺组织(P<0.05),且HPA和GABPα的表达与乳腺癌组织学分级、TNM分期、及淋巴结有无转移有关(P<0.05);Western blot检测显示HPA和GABPα蛋白表达在浸润性导管癌中明显高于正常乳腺组织(P<0.05),与免疫组化结果一致;两者在乳腺癌中的表达呈正相关(P<0.05)。结论在乳腺癌组织中,HPA与GABPα的表达均存在异常,提示HPA和GABPα在乳腺癌的发生发展、浸润转移中可能存在重要的协同作用。
Objective To analyze the clinicopathological significance of hepatanase（HPA） and GA-binding protein a （GABPa） in normal mammary glands and invasive ductal carcinoma （IDC） and to discuss the role and correlation between them during breast carcinggenesis. Methods HPA and GABPa expression were examined by immuneohistocberoistry,and the relationship between the HPA and GABPa protein expression and the clinical pathologic features were analyzed. Western blot analysis was conducted to determine the HPA and GABPa expression. Results Immunohistochemistry showed that heparanase and GABPa were distributed in cytoplasm and nuclear of tumor cells, in contrast to their nonexpression in normal breast tissue. GABP-positive cases and HPA-positive cases had a higher positive rate in breast cancer than that of normal breast tissues （P 〈 0.05 ）.As well as in western blot. The expressions of them were related to histological grade, TNM staging and lymph node metastasis. （P 〈 0.05）. And the expression of them were positively correlated in breast cancer （P 〈 0.05 ）. Conclusion Our results indicated that both SIAH2 and Bcl-2 are involved in breast cancer and might imply coordination in breast tumorigenesis.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2012年第1期52-55,58,共5页
Journal of China Medical University
