摘要
目的探讨Livin mRNA和Livin蛋白在脑胶质瘤中的表达及意义。方法采用逆转录聚合酶链反应(RT-PCR)和Western blot方法分别检测30例脑胶质瘤组织中Livinα、Livinβ的表达,分析其表达与胶质瘤临床病理特征的关系。结果30例胶质瘤组织标本中均可检测到Livin mRNA的表达,且Livin mRNA和Livin蛋白的表达明显高于正常脑组织,差异有统计学意义(P=0.008);Livin mRNA和Livin蛋白表达水平具有相关性(R=8.321,P=0.015)。且与胶质瘤细胞的分化程度、瘤周脑水肿密切相关。结论livin基因的表达水平上调与胶质瘤的发生发展密切相关。
Objective To study the expression of Livin and its clinicopathologic significance in glioma. Methods Western blot and reverse transcription-polymerase chain reaction （RT-PCR） were used for detecting the expression of Livin protein and mRNA in 30 specimens of glioma patients surgically treated from May 2004 to April 2005. The relationship between the expressions of Livin and the clinicopathological characteristics of glioma was analyzed. Results Expression of Livin mRNA could be detected in all 30 specimens and the expression level in glioma tissue （2. 13±0. 41） was significantly higher than that in normal brain tissue （0. 67±0. 13） （ P =0. 008）. In addition, the expression level of Livin mRNA was closely related with the expression of its protein. The expressions of Livin mRNA and protein were also correlated with differentiation degree of gliorna cell and brain edema around the tumor. Conclusion The up-regulation of Livin expression is closely correlated with pathogenesis of glioma.
出处
《中国耳鼻咽喉颅底外科杂志》
CAS
2007年第5期321-323,327,共4页
Chinese Journal of Otorhinolaryngology-skull Base Surgery
基金
国家自然基金项目(NO:30672149)
