摘要
目的探讨苦参碱作用人食管癌细胞株EC109后对细胞增殖的影响。方法应用不同浓度苦参碱(0.187 5、0.375、0.75、1.5mg/mL)作用体外培养人食管癌细胞株EC109后,分别用倒置显微镜观察细胞生长数量及其形态的变化;四甲基偶氮唑蓝(MTT)法检测苦参碱的细胞毒作用。结果不同浓度苦参碱作用人食管癌细胞株EC109后,细胞抑制率为15.60%～65.69%,与对照组比较细胞生长明显受到抑制(P<0.05)。且随着苦参碱浓度的增加及作用时间的延长,EC109存活细胞显著减少,呈明显的剂量依赖性。结论苦参碱能明显抑制人食管癌细胞株EC109细胞增殖。
Objective To investigate the effect of matrine on proliferation of EC-109 cell.Methods Ec-109 cells cultivated in vitro were treated by different concentrations of matrine（0.187 5,0.375,0.75,1.5 mg/mL）.And then,the changes of cell morphology and cell growth number were observed by inverted microscope and the cellulotoxic activity of matrine was detected by MTT.Results In the range of 0.187 5-1.5 mg/mL,the cells inhibition rate was 15.60%-65.69%,showing obvious inhibition of cell growth compared with the control group（P0.05）.With concentration increase and time extension,the survival EC-109 cells were significantly decreased,showing obvious dose dependence.Conclusion Matrine can significantly inhibit the proliferation of EC-109 cells in vitro.
出处
《检验医学与临床》
CAS
2012年第2期129-130,共2页
Laboratory Medicine and Clinic
基金
四川省卫生厅资助科研项目(100138)
关键词
苦参碱
食管癌细胞
增殖
matrine
esophagus carcinoma cell
proliferation
