摘要
利用硝态氮(NO3--N)、铵态氮(NH4+-N)和混合态氮对3个栽培大豆品种花荚期植株进行诱导处理,研究不同形态氮素对功能叶片硝酸还原酶(NR)、谷氨酰胺合成酶(GS)活性以及籽粒蛋白质含量的影响。结果表明,NH4+-N增加3个大豆品种功能叶片NR活性效果最好,其次是混合态氮,NO3--N效果较差;三种形态的氮素均能明显提高3个大豆品种功能叶片GS活性;在三种形态氮素诱导下,3个大豆品种的籽粒蛋白质含量均有不同程度的提高,且与其功能叶片NR和GS活性呈显著正相关(r=0.520*和0.550*);追施氮素对低蛋白大豆品种功能叶片NR、GS活性和籽粒蛋白质含量具有较好的促进作用。可以把大豆花荚期叶片NR和GS的活性作为高蛋白品种选育的参考指标之一。
Effects of different nitrogens (NO3-N, NH4+-N, NO3--NH,+) on nitrate reductase (NR) and glutamine synthetase (GS) activities of functional leaves, and seed protein contents of three soybean cultivars during flowering-poding phase were investigated. The results indicated that NR activities of functional leaves in three soybean genotypes treated by three kinds of nitrogens were enhanced, NH4+-N was the best, the second was NO3-NH4+, and the third was NO3-N. All three different nitrogen treatments could remarkably increase GS activities of functional leaves of three soybean cultivars. The seed protein content showed a very significantly positive correlation with the functional leaf NR activity and GS activity (r=0.520* and 0.550*). There is better promotion of nitrogen topdressing to activities of NR and GS in functional leaves and cotents of protein in seeds of the lower-protein soybean cultivar. Hence, it is suggested that high NR and GS activity in soybean functional leaves should be one of the bio-chemical index for selecting soybean germplasm with high protein.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2012年第1期31-35,共5页
Journal of Northeast Agricultural University
基金
福建省农业科技重大专项项目(2008NZ0002-1(3))
福建省财政专项(STIF-Y04)
福建省科技厅公益类科研院所专项(2011R1028-3)
农业部公益性行业(农业)科研专项(200903002)