摘要
氮代谢是植株体内最基本的物质代谢之一,硝酸还原酶是植物氮代谢的关键酶。主要对植物氮代谢在硝酸还原酶水平上调控的研究新进展,尤其是其合成/降解及活性调控机制进行了较为系统的综述。硝酸还原酶合成的调控主要发生在转录水平和翻译水平上,硝酸还原酶降解的调控主要发生在翻译后水平上,同时NO-3及光在硝酸还原酶转录水平调控上的作用重大,硝酸还原酶编码基因转录的mRNA的稳定性强弱影响植物的氮代谢,而影响mRNA稳定性的因素很多,机理复杂;磷酸化/去磷酸化在硝酸还原酶活性调控中占举足轻重的地位,研究也比较深入。钝化蛋白也能够影响硝酸还原酶活性,许多小分子物质对硝酸还原酶活性有影响。
Nitrate metabolism is one of the most important object turnovers.In the all pathways of nitrate metabolism,nitrate reductase is the key enzyme and it catalyzes the step from NO^-_3 to NO^-_2.NO^-_3 and light show great effects on the expression of gene that codes nitrate reductase.The mechanisms of the transcriptional and translational level must be involved in the regulation of nitrate reductase synthesis,and the mechanisms of posttranslational must be involved in the regulation of nitrate reductase degradation.The stability of nitrate reductase mRNA affects the activity of nitrate reductase smartly.The activity of nitrate reductase is also effected by phosphorylation/dephosphorylation.At the same time other mechanisms may play important role in the regulation of the activity of nitrate reductase and nitrate metabolism.
出处
《西北植物学报》
CAS
CSCD
2004年第7期1355-1361,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金资助项目(39770445)
关键词
植物
氮代谢
硝酸还原酶
调控机制
进展
plants
nitrate metabolism
nitrate reductase
regulation mechanism
advances
