摘要
目的考察肝经引经药醋柴胡水提液对大鼠肝细胞内pH值的影响,探讨引经药影响药物体内分布的可能机理。方法利用BCECF/AM和FITC-Dextran两种荧光探针,以空白细胞为参比,考察不同浓度药物在不同时间对细胞内pH的影响。结果药物对细胞质内pH值影响随时间、浓度不同而不同,24,48 h各药物剂量组细胞质中pH与空白组细胞相比有统计意义,其P<0.05,中浓度组作用3,18 h与空白组相比有显著差异,3 h高浓度组与空白组比较有统计学差异;加药组囊泡膜内pH值呈现下降的趋势,药物作用18,48 h囊泡膜内pH值与空白相比有统计意义,其P<0.01,高浓度组作用3,6,24 h与空白组相比有显著差异,3h低浓度组与空白组比较有统计学差异,其他各组细胞内及囊泡膜内pH值均与对照组没有显著差异,不同浓度组间无统计意义。结论醋柴胡可能通过改变大鼠肝细胞内pH值来影响药物体内分布。
Objective To investigate the effect of Traditional Chinese Medicine（TCM） meridine guide drug vinegar processing Radix Bupleuri on intracellular pH in BRL,and to approach the possible mechanism of that meridine guide drug influence in drug distribution in vivo. Methods Referenced by the normal BRL,the effect of different drug concentration in different time on intracellular pH of BRL was studied.The pHi was determined by fluorescent probe（BCECF/AM and FITC-Dextran）. Results The effect of drug on cytosolic（pHcyt） in BRL was different in different concentration or different time.Significant changes were found in BRL with 24h and drug effect（p0.05）,middle concentration drug have significant changes on pHcyt in 3h and 18h（p0.05）,high concentration drug in 3h.the drug has the tendency of descent apparent vesicular pH（pHves） in BRL.Significant changes were found in BRL with 18h and 48h drug effect（p0.01）,high concentration drug have significant changes on pHcyt in 3h,6h and 18h（p0.05）,low concentration drug in 3h.But no significant effect was found between different drug concentrations. Conclusion Intracellular pH in BRL may be affected by meridine guide drug vinegar processing Radix Bupleuri.The mechanism of vinegar processing Radix Bupleuri affect other drugs tissue distribution may be in the pathway.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2011年第12期2865-2867,共3页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(No.30672668)
关键词
醋柴胡
细胞内PH值
荧光法
Vinegar processing Radix Bupleuri
PHi
Fluorescence assay
