摘要
目的观察湿热型结肠炎中Th1/Th2的状态以及黄芩汤对Th1/Th2的影响,探讨其治疗湿热型结肠炎的免疫学机制。方法采用高脂、高糖饲料联合人工气候箱法造出大鼠湿热型体质,结合三硝基苯磺酸(TNBS)法建立湿热型结肠炎模型;将60只大鼠随机分为空白组、湿热+TNBS组、黄芩汤组、美沙拉嗪组;对大鼠进行临床活动度观察、结肠组织的宏观和微观观察、检测结肠长度重量、脾脏和胸腺的重量和髓过氧化物酶(MPO)的活性、采用酶联免疫吸附法(ELISA)分析大鼠血清和结肠组织中γ-干扰素(INF-γ)、白细胞介素12(IL-12)、白细胞介素4(IL-4)和白细胞介素10(IL-10)水平。结果黄芩汤能够有效缓解湿热型溃疡性结肠炎大鼠临床症状,降低大鼠血清及结肠组织Th1细胞因子INF-γ和IL-12并提高Th2细胞因子IL-4和IL-10的表达。结论黄芩汤可以改善湿热型溃疡性结肠炎的炎症反应,调节Th1/Th2细胞的平衡,这可能是其治疗湿热型溃疡性结肠炎的作用机制之一。
Objective To investigate the expression of Th1/Th2 profile in TNBS-induced colitis with damp-heat syndrome in rats and explore the therapeutic effects and the immunology mechanism of Huang-Qin-Tang. Methods The rat model of colitis with damp-heat syndrome were induced by giving high sugar fat food and put into an artificial bioclimatic chamber for 4 weeks and then rendered in rats by a single colonic administration of trinitrobenzene sulfonic acid（TNBS）.The experimental animals were randomly divided into damp-heat syndrome＋TNBS group,Huang-Qin-Tang treatment group,mesalazine treatment group and normal control group.Assessment of colitis severity was performed daily.Colon tissue were analyzed macroscopically and microscopically,and myeloperoxidase（MPO） activity,colon lengths and spleen and thymus weights were measured,as well as cytokine levels interferon-gamma,interleukin（IL）-12,interleukin（IL）-10,interleukin（IL）-4 in serum and colon tissue were determined by enzyme-linked immunosorbent assay. Results Compared with damp-heat syndrome＋TNBS group,Huang-Qin-Tang ameliorated TNBS-induced colitis with damp-heat syndrome by improved body weight and colon inflammation and tissue MPO accumulation.Th1-related parameters INF-γ and IL-12 were down-regulated,whereas Th2 markers lilke IL-4 and IL-10 were up-regulated. Conclusion These data confirm that Huang-Qin-Tang exerted therapeutic effects on colitis with damp-heat syndrome by regulating the shift from Th1 to Th2 profile.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2011年第11期2608-2611,共4页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(No.30772701)
东莞市科技计划项目(No.Q2007016)
