摘要
目的:探讨高迁移率族蛋白1(HMGB1)能否激活类风湿性关节炎滑膜成纤维细胞(RASF)表达基质金属蛋白酶-13(MMP-13)。方法:HMGB1与RASF共孵育后,采用流式细胞术、实时RT-PCR、ELISA分别检测细胞周期和MMP-13的基因表达量以及蛋白表达水平。结果:1μg/ml HMGB1刺激滑膜成纤维细胞3小时,MMP-13 mRNA的表达明显增加;HMGB1刺激滑膜成纤维细胞48小时后MMP-13蛋白分泌增高,并且滑膜成纤维细胞增殖周期速度加快。结论:HMGB1可以激活滑膜成纤维细胞,使其增殖周期速度加快,MMP-13表达增加,提示HMGB1通过作用于滑膜成纤维细胞表达MMP-13加强关节结构破坏。
Objective：To investigate whether high mobility group box chromosomal protein 1（HMGB1） can activate synovial fibroblasts to secrete matrix metalloproteinase-13.Methods：Synovial fibroblasts were cultured with HMGB1 for indicated period,then the mRNA and protein expression of matrix metalloproteinase-13 and proliferation rate were examined by real-time PCR,ELISA,and flow cytometric analysis.Results：Three hours after culture with HMGB1（1 μg/ml）,matrix metalloproteinase-13 mRNA expression was greatly increased.The expression of matrix metalloproteinase-13 protein was also greatly increased 48 h after culture with HMGB1,and synovial fibroblasts proliferation rate was increased.Conclusion：HMGB1 can directly activate synovial fibroblasts to secrete matrix metalloproteinase-13,suggesting that HMGB1 through express MMP-13 that take part in the occurrence or development course of RA.It plays an important role in inflammatory cell transforming and aggregating to synovial tissue.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2011年第12期1097-1100,共4页
Chinese Journal of Immunology
基金
国家自然科学基金资助项目(30972729
31000395)
