摘要
目的:研究乙肝疫苗接种者CD4+T细胞TCR Vβ基因克隆化特征,分析乙肝疫苗有应答者和无应答者TCR Vβ基因单克隆改变的差异性。方法:采用多引物PCR技术扩增80例乙肝疫苗接种者CD4+T细胞TCR Vβ基因22个家族的CDR3区基因片段,对PCR产物分别应用琼脂糖凝胶电泳和基因扫描两种方法检测。结果:80例乙肝疫苗接种者中有58例产生抗体,有22例未产生抗体。TCR Vβ基因单克隆改变主要集中在Vβ2、Vβ8、Vβ9、Vβ11和Vβ17五个家族上,乙肝疫苗无应答组这五个Vβ基因单克隆改变频率明显低于乙肝疫苗有应答组(P<0.01或P<0.05)。结论:CD4+T细胞TCR Vβ基因克隆化改变是影响机体对乙肝疫苗免疫应答效果的重要因素之一。
Objective：To study the characteristics of CD4＋ T cells TCR Vβ gene clonning in hepatitis B vaccinated and the difference of TCR Vβ genes monoclonal changes between the clone of genes in responders and non responders to hepatitis B vaccine.Methods：CD4＋ T cells of 80 hepatitis B vaccinated individual Vβ genes CDR3 region fragments were amplified by multi-primer PCR amplification methods,PCR products were aplied on agarose gel electrophoresis and gene scanning by sequencing instrument.Results：From 80 cases of hepatitis B vaccinated individual,58 cases were positive for anti-HBs and 22 cases were negative.Monoclonal TCR Vβ gene changes were mainly concentrated in Vβ2,Vβ8,Vβ9,Vβ11,and Vβ17 family of genes.The frequency of changes in these five family of genes was significantly lower in nonresponders group than responders（P0.01 or P0.05）.Conclusion：CD4＋ T cells of TCR Vβ gene change is one of the important factor that affect the body＇s immune response to hepatitis B vaccine.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2011年第12期1059-1061,1065,共4页
Chinese Journal of Immunology
基金
国家自然科学基金(No.30772026)
吉林省科技发展计划(20090942)资助项目
关键词
乙肝疫苗
T细胞抗原受体
免疫应答
Hepatitis B vaccine
T cell antigen receptor（TCR）
Immune response
