摘要
目的探讨系统性红斑狼疮(SLE)患者外周血中T细胞受体β链可变区(TCRBV)亚家族互补决定区3(CDR3)基因表达谱型及对增殖的T细胞克隆进行序列分析,了解克隆扩增的T细胞与自身免疫病的相关性。方法应用RT-PCR的方法扩增8例SLE患者外周血的TCRBV24个家族的基因序列,分析TCRBVCDR3基因表达情况。对增生的T细胞克隆的TCRBV进行序列分析,通过生物信息学分析,了解TCRBV基因与自身免疫病的相关性。结果8例SLE患者TCRBVCDR3谱型图均出现异常,表现为CDR3多态性降低,BV家族寡克隆增生。在TCRBV13.1基因家族有4例患者均出现寡克隆增生;在BV8、BV9、BV18家族中有3例出现寡克隆增生。未发现SLE的TCRBV的CDR3区有共同的氨基酸基序,但与基因库中的其他自身免疫病的T细胞克隆有较高的一致性。结论SLE患者T细胞出现寡克隆增生,CDR3多态性降低,可能与自身免疫病发病有关。
Objective To explore the T-cell recepter B variable （TCRBV） subfamily clonal repertoire in patients with systemic lupus erythrematosus（SLE） and obtained nucleotide sequences from expanded T cell clone and understand the association with other ilnrnune diseases. Methods Among 8 patients with SLE, 24 subfamilies of TCRBV were amplified using RT-PCR to examine the clonal repertoire of TCR CDR3 of T lymphocytes from peripheral blood. Nucleotide sequences were obtained from the CDR3 of the TCRBV gene in clone expanded T cell to analysis the association with the publiced CDR3 queries in immune diseases. Results Abnormal TCRBV families were showed in all 8 patients, oligoclonality emerged in some BV families. The TCR BV8, BV9, BV18 genes were preferentially expressed in 3 patients, and BV13. 1 gene was over-expressed in 4 patients and CDR3 length polymorphisms were decreasd. There were no the conserved TCRBV CDR3 amion acid motifs in SLE patients, but there was a high consistence with other immune diseases. Conclusion The decreases in T cell oligoclonality and CDR3 polymorphisms can be seen in SLE patients, which may be related to the pathogenesis of autoimmune disease.
出处
《江苏医药》
CAS
CSCD
北大核心
2007年第4期325-327,共3页
Jiangsu Medical Journal
关键词
系统性红斑狼疮
T细胞受体
互补决定区3
Systemic lupus erythematosus
T cell receptor
Complementarity determining region 3
