摘要
采用荧光定量PCR方法,研究了生物有机肥对盆栽烟草根际青枯病原菌和拮抗菌动态变化的影响。结果表明:施用生物有机肥显著降低了抗病品种‘K326’和感病品种‘南江三号’烟草青枯病的发病率,促进了烟草生长,生物量增加14%以上;只接种病原菌处理(RS)土壤中青枯病原菌的fliC基因拷贝数显著高于先接种病原菌再施用生物有机肥处理(RS+BIO)和先施用生物有机肥后接种病原菌处理(BIO+RS),且这种效应与生育期和品种无关;旺盛生长期前,施用生物有机肥处理土壤中短短芽孢杆菌16S rDNA基因拷贝数也显著高于RS处理。上述结果表明,施用生物有机肥防治烟草青枯病主要是通过抑制土壤青枯菌的增长和促进植物生长来实现的。
Pot experiments were carried out to quantify the effect of bio-organic fertilizer（BIO）on the population of Ralstonia solanacearum（RS）and Brevibacillus brevis in tobacco rhizosphere with the method of real-time PCR.The application of the BIO not only reduced the disease incidence of tobacco bacterial wilt in resistant variety ’K326’ and susceptible variety ’Nanjiang 3’,but also significantly promoted the growth of tobacco with higher than 14% of biomass increment.The real-time PCR results showed that the copy numbers of fliC gene in tobacco rhizosphere treated with RS was up to 107-108 g-1 dry soil,which was much higher than that in the treatments with BIO.The 16S rDNA copy numbers of Brevibacillus brevis was up to 106 g-1 dry soil in the treatments with BIO,while only 105 g-1 dry soil in the control.The results indicated that the bio-organic fertilizer enriched with Brevibacillus brevis could reduce the number of the pathogen in tobacco rhizosphere,thus effectively controlling the tobacco wilt disease and promoting the growth of tobacco.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2012年第1期75-79,共5页
Journal of Nanjing Agricultural University
基金
中国烟草总公司重点项目(11020100219)
贵州省烟草总公司重点项目(201018)
