摘要
目的观察功能化碳纳米管作为新型载体在胰腺癌淋巴化疗中的应用价值。方法将多壁碳纳米管功能化(胺基化),在透射电子显微镜下观察功能化碳纳米管进入人胰腺癌细胞株BxPC-3的情况。采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)实验检测功能化碳纳米管对BxPC-3细胞的毒性以及携带吉西他滨的功能化碳纳米管对BxPC-3细胞生长的影响。采用大鼠淋巴结示踪实验评价功能化碳纳米管的亲淋巴系统特性。结果在透射电子显微镜下观察发现,功能化的多壁碳纳米管的分散性明显增加,并可穿过细胞膜进入胰腺癌BxPC-3细胞。MTT实验检测发现,不同浓度的功能化碳纳米管对胰腺癌细胞BxPC-3的生长无明显影响;携带吉西他滨的功能化碳纳米管对BxPC-3细胞有明显的生长抑制作用,细胞抑制率为(50.2±5.0)%,略高于单用吉西他滨的(41.4±6.6)%,差异无统计学意义(P>0.05)。将碳纳米管注射入大鼠脚垫皮下。在其淋巴引流路径上的不同部位均可见到淋巴结黑染,且随淋巴流动分布范围较远,可达到大鼠肾门和腹主动脉旁淋巴结。结论功能化碳纳米管具有较好的亲淋巴系统特性,并有良好的药物携带能力和肿瘤细胞靶向性,载体本身对细胞无明显毒性作用,功能化碳纳米管可能成为新型的安全高效的胰腺癌淋巴系统药物载体。
Objective To investigate the application of functiona n lymphatic chemotherapy for pancreatic cancer. Methods The zed carbon nanotubes as a new carrier multi-walled carbon nanotubes were functionalized （aminated）. Then transmission electron microscopy （TEM） was used to observe the entrance of aminated carbon nanotubes into human pancreatic cancer cell line BxPC-3. The toxicity of aminated carbon nanotubes on BxPC-3 and the efficacy of carbon nanotubes carrying gemcitabine on cell growth were evaluated by 3-E4,5-Dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide （MTT） assay. And lymph node mapping was used to evaluate pro-lymphatic properties of carbon nanotubes in rats. Results TEM revealed an increased dispersion of multi-walled carbon nanotubes, which passed through the cell membrane into BxPC-3 cells. MTT assay showed no significant difference among the efficacy of different concentrations of carbon nanotubes on BxPC-3 cell growth, while the carbon nanotubes carrying gemcitabine significantly inhibited cell growth, slightly higher than gemcitabine group （50.2%±5.0% vs. 41.4%±6.6%, P〉0.05）. After subcutaneous injection in the foot pad of rats, nanotubes were tracked in various lymphatic drainage pathways （black dyed lymph nodes） with widespread distribution （as far as renal hilum and para-aortic lymph nodes）. Conclusion Functionalized carbon nanotubes are pro-lymphatic and competent of carrying drugs and targeting tumor cells, while the carrier itself shows no significant cell toxicity. Functionalized carbon nanotubes may be a novel safe drug delivery system in pancreatic lymphatic chemotherapy.
出处
《上海医学》
CAS
CSCD
北大核心
2011年第11期840-843,F0004,共5页
Shanghai Medical Journal
基金
国家自然科学基金资助项目(81001058)
高等学校博士学科点专项科研基金资助项目(20100071120092)
2010上海市卫生局科研资助项目(JGY1001)
2010复旦大学附属肿瘤医院青年骨干启动基金资助项目(GG201003)
关键词
碳纳米管
胰腺癌
载体
淋巴化疗
Carbon nanotubes
Pancreatic cancer
Carrier
Lymphatic chemotherapy
