摘要
目的观察磁流体在交变磁场中的升温行为并探讨其作为热疗介质应用于小鼠胰腺癌细胞(mouse pancreaticcancer cell,MPC-83)的热疗效应。方法将不同浓度Fe3O4水基磁流体置于中频交变磁场中观察其升温情况。透射电子显微镜(transmission electron microscopy,TEM)观察磁性纳米颗粒被MPC-83吞噬的情况,并将其置于交变磁场中升温至44℃,同时与兔平滑肌细胞(smooth muscle cell,SMC)比较,流式细胞仪检测细胞凋亡。结果在交变磁场中磁流体迅速升温,且升温与磁流体的浓度呈显著正相关关系(n=5,r=0.937,P=0.019)。透射电镜下(×120000)观察磁性纳米颗粒可被MPC-83吞噬。磁感应热疗后流式细胞仪检测MPC-83凋亡率为(26.87±8.50)%与SMC(18.45±3.28)%无统计学差异(n=3,t=1.601,P=0.185)。结论肿瘤治疗用磁流体可以在交变磁场中产热,通过改变磁流体浓度可进行温度控制,小鼠胰腺癌细胞对磁性纳米颗粒有较强的吞噬能力,且对温度升高比正常细胞敏感。
Objective To determine the heating ability of magnetic fluid in alternating magnetic field in vitro and its application to hyperthermia for pancreatic cancer in mice （ MPC-83 cells）. Methods Different concentrations of magnetic fluid were prepared in vitro, and heated for 30 min in an intermediate frequency alternating magnetic field. After the phagocytosis phenomenon was observed by transmission electron microscopy （TEM） , MPC-83 ceils were placed in an intermediate frequency alternating magnetic field with the temperature raised to 44℃. Apoptosis of the MPC-83 was quantitatively measured with flow cytometry, and the results were compared with that of smooth muscle cells （SMCs） obtained from rabbits. Results The temperature of magnetic fluid raised rapidly in the alternating magnetic field. The increase of the temperature was positively correlated to the concentration of magnetic fluid （ n = 5, r = 0. 937, P = 0. 019）. TEM （ x 120 000） showed that the magnetic nanoparticles were phagocytosed by the MPC-83 cells; meanwhile flow cytometry revealed an apoptosis rate of （26. 87 ± 8.50）% in the MPC-83 cells, which was significantly higher than that in the SMC [（18.45 ±3.28）%, n=3, t =1.601,P=0.185）. Conclusions Magnetic fluid used for tumor therapy has a good heating ability in an alternating magnetic field in vitro and its temperature can be controlled by changing the concentration of the magnetic fluid. Magnetic nanoparticles can be simultaneously phagocytosed by MPC-83 cells, which is more sensitive to hyperthermia than normal cells.
出处
《中国微创外科杂志》
CSCD
2009年第3期246-249,共4页
Chinese Journal of Minimally Invasive Surgery
基金
国家自然科学基金(10775085)
国家自然科学基金(30571799)
北京市科委(Z07000200540704)
清华大学裕元基金
关键词
小鼠胰腺癌细胞
平滑肌细胞
磁流体
交变磁场
热疗
Mouse pancreatic cancer cell
Smooth muscle cell
Magnetic fluid
Alternating magnetic field
Hyperthermia
