摘要
目的:建立测定大鼠血浆中丹红注射液中迷迭香酸、紫草酸、丹酚酸B浓度的方法。方法:采用HPLC法,色谱柱为Diamonsil C18柱(250 mm×4.6 mm5,μm);流动相为乙腈-0.4%的磷酸水,梯度洗脱;流速为1.0 mL.min-1;检测波长为288 nm。结果:迷迭香酸在4.0～100.0μg.mL-1浓度范围内线性关系良好(r=0.9925);回收率在85.0%～101.4%之间,日内、日间RSD<8%;紫草酸在4.0～100.0μg.mL-1浓度范围内线性关系良好(r=0.991 1);回收率在84.4%～93.9%之间,日内、日间RSD<9%;丹酚酸B在2.0～200.0μg.mL-1浓度范围内线性关系良好(r=0.998 6);回收率在80.0%～97.4%之间,日内、日间RSD<5%。结论:本文所建立的方法灵敏度好、准确率高,可用于丹红注射液中迷迭香酸、紫草酸、丹酚酸B的药代动力学研究。
Objective： To establish an HPLC method to determine the levels of rosmarinic acid,lithospermic acid and salvianolic acid B in rat plasma after iv administration of Danhong injection.Methods： In a Diamonsil C18 column,acetonitrile-0.4% phosphoric acid solution was used as the mobile phase,the flow rate was 1 mL·min-1,and detection wave length was at 288 nm.Results： Rosmarinic acid showed a good linear relationship in concentrations of 4.0~100.0 μg·mL-1（r=0.992 5）;absolute recoveries were between 85.0%~101.4%;the RSD of intra-and inter-day was less than 8%.Lithospermic acid showed a good linear relationship in concentrations of 4.0~100.0 μg·mL-1;absolute recoveries were between 84.4%~93.9%;the RSD of intra-day and inter-day was less than 9%.Salvianolic acid B showed a good linear relationship in concentrations of 2.0~200.0 μg·mL-1（r=0.998 6）;absolute recoveries were between 80.0%~97.4%;the RSD of intra-day and inter-day was less than 5%.Conclusion： The method is simple,quick and convenient for the pharmacokinetics of plasma rosmarinic acid,lithospermic acid and salvianolic acid B.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2011年第24期2475-2478,共4页
Chinese Journal of New Drugs
