摘要
目的建立夫西地酸钠原料高效液相色谱法含量测定的方法。方法采用HPLC法,色谱柱为Diamonsil((钻石)C18(250×4.6 mm5μm),流动相:0.05 mol/L磷酸-乙腈-甲醇(40∶50∶10);检测波长:235nm;流速:1.0 mL/min;柱温:30℃。结果夫西地酸钠的进样量在0.379～30.35μg范围内,与其峰面积有良好的线性关系(r=0.999 9),高中低3种浓度的加样回收率为99.34%～100.66%,相对标准差(RSD)为0.17%～0.76%。结论方法简便易行、准确可靠,可用于夫西地酸钠原料的含量测定。
Objective To establish the reversed phase high-performance liquid chromatography(RP-HPLC) assay for determination of the content of sodium fusidate for injection.Methods RP-HPLC assay was adopted.Diamonsil C18(250 mm×4.6 mm,5 μm) was chosen as the chromatographic column with UV detection wavelength at 235 nm,and the mobile phase consisting of 0.05mol/L phosphoric acid-acetonitrile-methanol(40∶50∶10) was used;the flow rate was 1.0 mL/min,and the column temperature was 30 ℃.Results The calibration curve was l...
出处
《中国医药指南》
2008年第18期55-,57,共2页
Guide of China Medicine
