摘要
目的:观察清热祛瘀颗粒对糖尿病Apo E基因敲除小鼠冠状动脉粥样硬化炎症反应的影响。方法:Apo E基因敲除小鼠随机分为糖尿病对照组、非糖尿病对照组和清热祛瘀颗粒组,分别给予清热祛瘀颗粒及生理盐水16周,评估冠状动脉主干的病理形态学改变及冠状动脉周围的炎症浸润程度,免疫组化法测定单核细胞/巨噬细胞-2(MOMA-2)表达、ELISA方法测定细胞间黏附分子(ICAM-1)表达、Real-time PCR的方法测定ICAM-1、白细胞趋化因子(MCP-1)、人血红素氧合酶(HO-1)的表达。结果:糖尿病对照组冠状动脉粥样硬化程度更加显著;清热祛瘀颗粒能显著降低冠状动脉粥样硬化程度及冠脉主干周围炎症,并显著降低ICAM-1、MCP-1、升高HO-1mRNA的表达。结论:清热祛瘀颗粒可显著抑制糖尿病动脉粥样硬化小鼠冠状动脉血管内、外膜的炎症反应及炎性因子的表达,减缓冠状动脉粥样硬化的进展。
Objective： To investigate the inflammatory reaction changes on coronary artery of Apo E knockout mice with diabetes mellitus by treating with Qingre Quyu Granule（QRQY）.Methods： Apo E knockout mice were randomly divided into diabetic group,QRQY group and no-diabetic group.They were given normal saline or QRQY Granule for 16 weeks.At the end of the study we evaluated the main coronary artery and inflammatory infiltration surrounding the coronary artery.We also measured the expression of ICAM-1,MCP-1 and HO-1 by Real-time PCR.Results： The degree of coronary artery atherosclerosis in the diabetic group was more serious than that of the no-diabetic group.The QRQY could inhibit the coronary artery atherosclerosis and inflammatory infiltration surrounding the coronary artery,reduce the MOMA-2 content,the expression of ICAM-1 and MCP-1 and could also improve the expression of HO-1.Conclusion： The QRQY Granule can inhibit the inflammation reaction of coronary artery,and slow down the progression of coronary artery atherosclerosis.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2012年第1期210-212,I0002,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
北京市首都医学发展科研基金(No.SF-2007-III-41)
中日友好医院领先学科基金(No.ZDXK-LX01-01)~~
