摘要
棉酚是棉花中倍半萜(十五碳)的法尼基焦磷酸环化后合成的次生代谢产物。近来有研究显示棉酚具有抗肿瘤效果。本研究通过MTT、Hoechst染色、Western blot等生化技术开展棉酚对肝癌细胞系Bel-7404的增殖抑制及促凋亡作用研究。MTT实验结果表明5mmol棉酚即对肝癌细胞增殖具有强烈抑制作用,Hoechst染色表明5mmol/L棉酚即可对肝癌细胞具有促凋亡作用,Western blot结果表明棉酚能下调Bcl-2基因的表达。棉酚对肝癌细胞系Bel-7404具有很好的抑制增殖和促凋亡的效果。
Gossypol is generated from sesquiterpene in cottons after thefarnesyl-pyrophosphoric is cyclized. For the past years there were evidences showing that the gossypol has the capacity of anti-tumor. The experiment studies the capacity of suppressing proliferation and promoting apoptosis in of gossypol in hepatoma cell line Bel-7404 via MTT Hoechst and West-blotting. The MTT shows that the gossypol has the activity of suppressing proliferation, the Hoechst shows that it promotes apoptosis in Bel-7404 and the Western-blot shows that it can reduce the expression of Bcl-2. The experiment offer the reference for the forward research and clinical application.
出处
《浙江理工大学学报（自然科学版）》
2012年第1期103-106,共4页
Journal of Zhejiang Sci-Tech University(Natural Sciences)
基金
国家自然科学基金项目(30872984)
关键词
棉酚
肝癌
凋亡
Bd-2
gossypol
hepatocarcinoma
apoptosis
Bcl-2
