摘要
目的研究临床痰液分离的产ESBLs肺炎克雷伯菌Ⅰ、Ⅱ类整合子分布情况,并进行基因分型。方法分离临床痰液中100株产ESBLs的肺炎克雷伯菌,用WHONET 5.4分析菌株药敏情况,PCR检测整合酶Ⅰ、整合酶Ⅱ,ERIC-PCR进行基因分型。结果 100株菌对碳青霉烯类敏感率100%,对β-内酰胺类、氨基糖苷类、氟喹诺酮类多数耐药。整合酶Ⅰ检出率为60%,未检出整合酶Ⅱ。100株菌分为72个基因型。结论Ⅰ类整合子广泛存在于产ESBLS肺炎克雷伯菌中,与肺炎克雷伯菌的耐药相关,ERIC-PCR可用于临床分离肺炎克雷伯的基因分型。
Objective To investigate the distribution of class Ⅰ and Ⅱimegrons in Klebsiella pneumoniae isolated from sputamentum and evaluate the genetic relatedness of these isolates. Method Drug resistance was analyzed by WHONET 5.4. ERIC-PCR was used for determination of the genotypes. Class Ⅰ and Ⅱ integrase genes were detected by PCR. Result The resistance to most beta-lactam antibiotic, aminoglyeosides and quinolones antibiotics were more serious. 60 （66%） strains were found containing class Ⅰ Integrase, while class ⅡIntegrase were not found. 100 strains of bacterial isolates contained 72 genotypes. Conclusion Class I integrons are widespread in Klebsiella pneumoniae isolates. ERICPCR can be a convenient and effective method for genotyping clinical Klebsiella pneumoniae isolates.
出处
《中国微生态学杂志》
CAS
CSCD
2011年第12期1121-1123,共3页
Chinese Journal of Microecology
