摘要
目的了解来源于不同临床标本的肺炎克雷伯菌的耐药性、携带第1类耐药整合子的特征及基因盒的种类。方法使用常规方法分离肺炎克雷伯菌;运用法国梅里埃VITEK细菌分析系统进行鉴定;应用PCR法扩增第1类整合子;应用纸片扩散法对22种抗生素进行耐药性监测和分析。结果 191株产ESBLs肺炎克雷伯菌中检测出Ⅰ类整合子178株,检出率为93.2%,Ⅰ类整合子阳性菌对氨基糖苷类、喹诺酮类及头孢菌素类药物表现出较高的耐药,其多重耐药率明显高于Ⅰ类整合子阴性菌株。结论Ⅰ类整合子广泛地存在产ESBLs肺炎克雷伯菌中,Ⅰ类整合子对细菌多重耐药性的产生和传播起着重要作用。
Objective To understand the resistance and the characteristic of class 1 integron carried by K.pneum oniae from different samples,and elucidate the status of gene cassettes.Methods Routine method was used to isolate K.pneum oniae;identified by V ITEK;Antibiotic suscep tibility was tested with the K-B diffusion method;Class 1 integrons were detected by PCR.Results ClassⅠintegron gene was found in 93.2% of 178 strains of ESBLs-producing Klebsiella pneum onia.The positive strains of classI integrons showed higher multidrug resistance to aminoglycosides and/or fluoroquinolones and /or cephalosporins than the negative strains of classI integrons.Conclusion ClassI integrons were p revalent in ESBLs-producing Klebsiella pneum onia and played an important role in the development and dissemination ofmultidrug resistance.
出处
《中国实验诊断学》
北大核心
2010年第7期1097-1099,共3页
Chinese Journal of Laboratory Diagnosis
