摘要
目的探索白藜芦醇对酒精诱导的心肌纤维化的作用及其分子机制。方法将雄性SD大鼠随机分为对照组、酒精组和酒精+白藜芦醇组,每组10只大鼠,干预6个月后取心肌行常规石蜡切片和Masson染色评估心肌纤维化,采用冰冻切片和免疫荧光法检测纤连蛋白的表达,采用免疫印迹法检测心肌组织中基质金属蛋白酶2和基质金属蛋白酶9的表达。结果 6个月酒精摄入可显著增加心肌间质纤维化并明显破坏纤连蛋白的结构,白藜芦醇能显著削弱酒精诱导的心肌纤维化和纤连蛋白破坏。酒精摄入可显著增加心肌组织中基质金属蛋白酶2和基质金属蛋白酶9的表达水平,而白藜芦醇能显著抑制基质金属蛋白酶2和基质金属蛋白酶9的表达水平。结论白藜芦醇能通过抑制基质金属蛋白酶2和基质金属蛋白酶9的表达来抗酒精诱导的心肌纤维化。
Aim To investigate the effect of resveratrol on alcohol-induced myocardial fibrosis and the underlying molecular mechanism. Methods Male Sprague-Dawley rats were divided into three groups： control group, alcohol group, and alcohol plus resveratrol group, 10 rats in each group. After the intervention for six months, the myocardial fibrosis was evaluated by Masson staining, the fibronectin expression in myocardium was evaluated by immunofluorescence, the expressions of matrix metalloproteinase （MMP） -2 and MMP-9 were determined by Western blot. Results Alcohol intake for six months caused a significant myocardial fibrosis and an obvious decrease in fibronectin. Oral administration with resveratrol partially reversed alcohol-induced myocardial fibrosis and fibronectin loss. Alcohol intake significantly in- creased the expressions of MMP-2 and MMP-9. Treatment with resveratrol remarkably attenuated alcohol-induced upregnlations of MMP-2 and MMP-9. Conclusion Resveratrol inhibits MMP-2 and MMP-9 and consequently prevents alcoholic myocardial fibrosis.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2012年第1期21-24,共4页
Chinese Journal of Arteriosclerosis
基金
成都军区医学科研"十一五"课题(MB09023)
关键词
白藜芦醇
酒精性心肌纤维化
基质金属蛋白酶
Resveratrol
Alcohol-Induced Myocardial Fibrosis
Matrix Metalloproteinase
