摘要
目的:应用RNA干扰技术抑制促血管形成因子基因的表达,观察结肠癌细胞增殖和凋亡的变化及对结肠癌肿瘤生长的影响,为进一步的临床应用研究提供理论基础。方法:利用60只裸鼠建立肿瘤动物模型,并随机分成5组。通过移植瘤内注射pshRNA-Del1和pshRNA-VEGF载体质粒,观察并比较pshRNA-Del1治疗组、pshRNA-VEGF治疗组和联合治疗组对裸鼠移植瘤生长的影响;通过免疫组化法检测肿瘤组织中Ki-67的表达和微血管密度,进一步探讨基于RNA干扰的治疗对结肠癌疗效的影响。结果:RNAi通过抑制基因VEGF和Del1的表达,能显著抑制结肠肿瘤的生长,减低肿瘤的血管密度,减弱肿瘤细胞增殖。结论:协同阻断基因VEGF和基因Del1的表达能显著抑制结肠肿瘤的生长,并在结肠癌的治疗方面具有明显的作用。
Objective：To observe cell proliferations and apoptosis of colon cancer after inhibiting pro-angiogenic factor gene by the way of RNA interference,and hope that the study would provide a theoretical basis on the further studied in the future.Methods：Animal model was established by using 60 nude mice,which were divided into 5 groups randomly.By injecting pshRNA-Del1 and pshRNA-VEGF plasmid to node mice,the effects of treatments on tumor growth among pshRNA-Del1 treatment group,pshRNA-VEGF treatment group and combined treatment group were observed.Furthermore,in order to explore the efficacy of the treatment of colon cancer,the expression of Ki-67 and the density of micro-vessel by immunohistochemical staining of tumor tissue were detected.Results：On the one hand,RNA interference had the ability to inhibit the expression of VEGF and Del1 to significantly inhibited the growth of colon cancer.On the other hand,the RNAi treatment not only reduced densities of tumor blood vessels but also decreased the proliferations of tumor cells.Conclusion：Blocking the gene expression of VEGF and Del1 together can significantly inhibit the growth of colon cancer.Meanwhile,this kind of RNAi treatment has a significant role in therapeutics of colon cancer.
出处
《中国医药导报》
CAS
2011年第36期33-37,共5页
China Medical Herald
基金
黑龙江省教育厅科学技术研究项目(项目编号:11551207)
