摘要
本试验就超声波法提取大黄多糖工艺,以时间、温度、料液比和pH值4个因素进行单因素观察,在此基础上进行正交设计优化提取工艺,并对纯化后大黄多糖进行抗新城疫病毒活性试验。结果显示,超声波提取大黄多糖的最佳工艺条件是时间50min,温度40℃,料液比1∶40,pH值9.0,提取率及多糖含量为42.27%和13.11%,显著高于传统法的38.10%与5.06%,且提取率及含量稳定性高。体外抗病毒试验显示,两种作用方式下,治疗指数均可达4,说明大黄多糖具有较强抗新城疫病毒作用。
The study on ultrasonic extracting way of Rheum tanguticum polysaccharide （RTP） was carried out based on the extraction time, temperature, material--fluid ratio, and pH value as the single factor, respectively. And then orthogonal design was used to optimize the extraction way. Finally, the antiviral activity of purified RTP against NDV was analyzed. Our results showed that the optimal conditions are. 50 min, 40℃; material-fluid ratio 1 ：40, and pH 9.0. Using the optimal extraction condition, the ratio and the content of RTP were 42.27%, and 13.11%, respectively, which was significantly increased when compared with the number 38. 10%, and 5. 06% gained by traditional way. In-vitro experiment showed that the therapeutic index reached 4, indicating that RTP had anti-NDV activity.
出处
《中国兽医杂志》
CAS
北大核心
2011年第12期70-73,共4页
Chinese Journal of Veterinary Medicine
基金
黑龙江对外合作项目(wb08b01)
