摘要
目的探讨检测≥80岁老年恶性肿瘤患者外周血调节性T细胞(Treg)的临床意义。方法应用流式细胞学方法和免疫比浊法分别检测≥80岁健康老年人30例(对照组)及恶性肿瘤患者70例外周血Treg比率和血清C-反应蛋白(CRP)浓度,同时根据国际抗癌协会标准进行临床分期,根据Karnofsky法评定患者功能状态。结果肿瘤组外周Treg比率、血清CRP浓度与对照组比较明显升高,差异具有统计学意义(P均<0.01)。直线相关分析显示,肿瘤组外周血Treg比率与血清CRP浓度、肿瘤TNM临床分期正相关(r=0.416、0.248,P<0.01),与KPS评分负相关(r=-0.438,P<0.01)。结论≥80岁老年恶性肿瘤患者外周血Treg比率明显升高,且与CRP浓度、TNM临床分期及KPS评分具有一定的相关性。检测≥80岁老年恶性肿瘤患者外周血Treg比率有助于判断预后。
Objective To evaluate the change of CD4＋CD25＋regulatory cells（Treg） in peripheral blood of patients with malignant tumor aged 80 years old and over.Methods Seventy elderly patients with malignant tumor aged 80 and over were enrolled in this study,and 30 healthy aged people were enrolled as controls.The ratio of Treg in the peripheral blood was determined by flow cytometry.Meanwhile,the plasma level of C-reactive protein（CRP） was detected by immunoturbidimetry.Results The ratio of Treg in the peripheral blood and the plasma levels of CRP in very elderly patients with malignant tumor were significantly higher than those in the normal controls（P〈0.01）.With the increase of the level of CRP and tumor clinical stage,the ratio of Treg in the peripheral blood increased.There were significant positive correlations between the ratio of Treg in the peripheral blood and the plasma levels of CRP,tumor clinical stage.Reversely,there was a significant negative correlation between the ratio of Treg and Karnofsky score.Conclusions The ratio of Treg in the peripheral blood in very elderly patients with malignant tumor is significantly increased in comparison with that in healthy subjects.It helps determine the prognosis of very elderly patients with malignant tumor by detecting the ratio of Treg in the peripheral blood.
出处
《实用老年医学》
CAS
2011年第6期451-453,共3页
Practical Geriatrics
基金
江苏省卫生厅课题(P200916)
